Sep 1, 1994

Developmental expression of the sea urchin egg receptor for sperm

Developmental Biology
K OhlendieckWilliam J Lennarz

Abstract

Little is known about the biochemical changes underlying the morphological differentiation of the sea urchin egg during oogenesis. Because of this and the essential role of gamete recognition in fertilization, we studied the developmental expression of the recently identified egg surface receptor for sperm during oogenesis. Consecutive stages of ovaries undergoing oogenesis over a 4-month time course were examined morphologically and assessed with respect to content of sperm receptor mRNA, as well as the content and subcellular distribution of the sperm receptor glycoprotein. Although in early oocyte stages neither mRNA encoding for the receptor nor receptor glycoprotein was detectable, at the last two stages of development the level of receptor mRNA accumulation increased dramatically. This finding correlated well with immunoblot analyses which established that sperm receptor protein was only detectable at the last two stages of egg maturation. Interestingly, immunocytochemistry showed that the formation of the receptor correlated temporally and spatially with the formation of cortical granules. In the earlier of these two stages of maturation, the receptor population identified by immunoblotting was found by immunocytochemist...Continue Reading

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Mentioned in this Paper

ZP3 gene
Microvilli
Both Ovaries
Hormone Receptors, Cell Surface
Glycoproteins
Ovary
Biologic Development
Gamete Recognition
Immunocytochemistry
Immunoblotting

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