Developmental expression of the zebrafish Arf-like small GTPase paralogs arl13a and arl13b

Gene Expression Patterns : GEP
Ping Song, Brian D Perkins

Abstract

Members of the Arf-like (Arl) family of small GTP-binding proteins regulate a number of cellular functions and play important roles in cilia structure and signaling. The small GTPase Arl13a is a close paralog to Arl13b, a small GTPase required for normal cilia formation that causes Joubert Syndrome when mutated. As mutation of arl13b causes a slow retinal degeneration in zebrafish (Song et al., 2016), we hypothesized that expression of arl13a may provide functional redundancy. We determined the expression domains of arl13a and arl13b during zebrafish development and examined subcellular localization by expression of fluorescence fusion proteins. Both genes are widely expressed during early cell division and gastrulation and Arl13a and Arl13b both localize to microtubules in ciliated and dividing cells of the early zebrafish embryo. Between 2 and 5 days post fertilization (dpf), arl13b is expressed in neural tissues while expression of arl13a is downregulated by 2 dpf and restricted to craniofacial structures. These results indicate that arl13a and arl13b have evolved different roles and that arl13a does not function in the zebrafish retina.

References

Apr 24, 2001·Methods : a Companion to Methods in Enzymology·T Jowett
Apr 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian D PerkinsJohn E Dowling
Mar 28, 2006·The American Journal of Pathology·Jeffrey J SchrickDennis S Rice
Oct 3, 2006·Annual Review of Physiology·Peter Satir, Søren Tvorup Christensen
May 10, 2007·Developmental Cell·Tamara CasparyKathryn V Anderson
Sep 1, 2007·Molecular Biology of the Cell·Narendra PathakIain A Drummond
Apr 1, 2008·Nature Structural & Molecular Biology·Stefan VeltelAlfred Wittinghofer
Aug 5, 2008·American Journal of Human Genetics·Vincent CantagrelJoseph G Gleeson
Jan 17, 2009·Current Topics in Developmental Biology·Neeraj SharmaBradley K Yoder
Jun 6, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sudipto Roy
Jun 12, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shannon C LuntBrian D Perkins
Mar 23, 2010·Nature Cell Biology·Antonia BorovinaBrian Ciruna
Jun 10, 2010·The Journal of Cell Biology·Yujie LiJinghua Hu
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Hiroaki Ishikawa, Wallace F Marshall
Mar 6, 2012·Journal of Cellular Biochemistry·Yujie LiJinghua Hu
Nov 23, 2012·The Journal of Comparative Neurology·Michelle Ramsey, Brian D Perkins
Apr 4, 2013·Experimental Cell Research·Qing ZhangKun Ling
May 15, 2013·Nucleic Acids Research·Hamish McWilliamRodrigo Lopez
Oct 3, 2015·Advances in Experimental Medicine and Biology·Christin Hanke-GogokhiaWolfgang Baehr
Jan 28, 2016·The Journal of Biological Chemistry·Christin Hanke-GogokhiaWolfgang Baehr
Mar 2, 2016·Current Opinion in Cell Biology·Keren I HilgendorfPeter K Jackson
Mar 5, 2016·Human Molecular Genetics·Zachary C WrightVisvanathan Ramamurthy
Aug 30, 2016·Investigative Ophthalmology & Visual Science·Ping SongBrian D Perkins
Sep 17, 2016·Trends in Cell Biology·Jarema J Malicki, Colin A Johnson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The American Journal of Gastroenterology
Rena YadlapatiBruce K Tan
Methods in Molecular Biology
Mohammad Reza AhmadianChristian Herrmann
Small GTPases
Cristina CasalouDuarte C Barral
© 2022 Meta ULC. All rights reserved