Developmental expression of three genes for larval cuticular proteins of the tobacco hornworm, Manduca sexta

Developmental Biology
L M RiddifordJ E Rebers

Abstract

Three cDNA clones coding for the 12.8, 13.3, and 14.6 kDa larval cuticular proteins of the tobacco hornworm, Manduca sexta, were isolated and characterized. Hybridization to abdominal epidermal RNA from different stages showed that the genes for the 12.8 and 13.3 kDa proteins were expressed only during larval life. By contrast, the gene for the 14.6 kDa protein was expressed throughout the segment during the feeding, growing larval stages, then only in the flexible intersegmental regions during the deposition of endocuticle in the pharate pupa and adult. Quantitative RNA dot blot hybridizations showed that the RNA for each protein disappeared during the larval molt when the ecdysteroid titer was high, then reappeared during the preecdysial deposition of endocuticle. All disappeared when the epidermis became pupally committed at the onset of wandering. Exposure of the fourth instar epidermis to 20-hydroxyecdysone (20HE) in vitro under conditions that lead to the formation of a new larval cuticle by 48 hr caused the disappearance of these RNAs by 18 hr. Exposure of Day 2 fifth instar epidermis to 20HE in vitro caused a depression of these RNAs which in the case of the RNAs coding for the 12.8 and 13.3 kDa proteins was partially p...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R P RicciardiB E Roberts
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Mar 1, 1985·Annals of Internal Medicine·J H Oppenheimer
Aug 1, 1985·Developmental Biology·K Hiruma, L M Riddiford
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Mar 1, 1981·General and Comparative Endocrinology·A C Chen, L M Riddiford
Feb 1, 1982·Developmental Biology·C J ChiharaJ W Fristrom
Mar 1, 1984·Developmental Biology·T E Crowley, E M Meyerowitz
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jun 1, 1984·The Journal of Experimental Zoology·K Hiruma, L M Riddiford
Sep 1, 1983·Developmental Biology·L M Schwartz, J W Truman
Jun 1, 1982·Developmental Biology·J W FristromD J Silvert
Jul 1, 1981·General and Comparative Endocrinology·W E BollenbacherL I Gilbert

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Citations

Jan 1, 1996·Archives of Insect Biochemistry and Physiology·L M Riddiford
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·L M RiddifordB A Webb
Feb 1, 1995·Insect Biochemistry and Molecular Biology·M R KanostE Willott
May 1, 1996·Insect Biochemistry and Molecular Biology·B M Okot-KotberK J Kramer
Jun 9, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anna Wynn, Thomas H Shafer
Jun 5, 2003·Developmental Biology·Geoffrey E StilwellLynn M Riddiford
Mar 1, 2003·Insect Biochemistry and Molecular Biology·R J SudermanK J Kramer
Jul 25, 2003·Insect Biochemistry and Molecular Biology·Jianguang DuLynn M Riddiford
Mar 1, 1997·Insect Biochemistry and Molecular Biology·J E RebersL M Riddiford
Jan 26, 2000·Insect Biochemistry and Molecular Biology·S MissiosJ S Doctor
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Seong Ryul KimByung Rae Jin
Jul 6, 2004·Journal of Insect Physiology·Que Lan, Christopher A Grier
Jul 2, 1998·Journal of Invertebrate Pathology·B A KirkpatrickL E Volkman
Aug 16, 2016·Insect Biochemistry and Molecular Biology·Michael R KanostGary W Blissard
Sep 20, 1988·Journal of Molecular Biology·J E Rebers, L M Riddiford

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