Developmental-form-specific DNA-binding proteins in Chlamydia spp.

Infection and Immunity
E A Wagar, R S Stephens

Abstract

We identified DNA-binding proteins specific to the elementary body (EB) developmental form of Chlamydia spp. Chlamydial proteins from whole lysates were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with nick-translated chlamydial DNA. By this method, C. trachomatis serovar L2 EBs had three unique protein bands of 58,000, 25,700, and 17,000 molecular weight not seen in the reticulate bodies. The 17,000-molecular-weight protein and the 25,700-molecular-weight protein were identified in an acid-soluble protein fraction and were resistant to high-salt elution, similar to other procaryotic nucleoproteins. The 17,000-molecular-weight protein was also detected in preparations with isolated chromosomes from EBs. Preliminary characterization indicated that the protein-DNA interaction was principally charge related.

References

Jul 23, 1975·Biochimica Et Biophysica Acta·D G Searcy
Feb 23, 1978·The New England Journal of Medicine·J Schachter
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Rouvière-Yaniv, F Gros
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C M SilvaJ A Cidlowski
Sep 1, 1987·Microbiological Reviews·K Drlica, J Rouviere-Yaniv
Aug 1, 1986·Journal of Bacteriology·W G WeisburgC R Woese
Jul 17, 1986·The New England Journal of Medicine·J T GraystonJ Altman
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Mar 15, 1983·European Journal of Biochemistry·B LaineP Sautière
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R LatheE K Bautz
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U HübscherA Kornberg
Jan 1, 1980·Annual Review of Microbiology·J Schachter, H D Caldwell

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Citations

Sep 1, 1990·Molecular Microbiology·M V SolbrigR S Stephens
Mar 1, 1993·Molecular Microbiology·S A MathewsT P Hatch
Mar 15, 1992·FEMS Microbiology Letters·E VretouR Kouvatsou
Aug 1, 1989·Journal of Bacteriology·D W Dorward, C F Garon
Dec 1, 1992·Journal of Bacteriology·N G Magill, P Setlow
Mar 1, 1993·Journal of Bacteriology·G ChristiansenS Birkelund
Aug 1, 1996·Journal of Bacteriology·R A Heinzen, T Hackstadt
May 1, 1993·Journal of Bacteriology·D G Wichlan, T P Hatch
Mar 1, 1991·Microbiological Reviews·J W Moulder
May 1, 1994·Trends in Microbiology·G McClarty

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