PMID: 8601585Mar 1, 1996Paper

Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans

The Journal of Cell Biology
C D MyersT Bogaert

Abstract

We have been investigating a set of genes, collectively called mups, that are essential to striated body wall muscle cell positioning in Caenorhabditis elegans. Here we report our detailed characterization of the mup-2 locus, which encodes troponin T (TnT). Mutants for a heat-sensitive allele, called mup-2(e2346ts), and for a putative null, called mup-2(up1), are defective for embryonic body wall muscle cell contraction, sarcomere organization, and cell positioning. Characterizations of the heat-sensitive allele demonstrate that mutants are also defective for regulated muscle contraction in larval and adult body wall muscle, defective for function of the nonstriated oviduct myoepithelial sheath, and defective for epidermal morphogenesis. We cloned the mup-2 locus and its corresponding cDNA. The cDNA encodes a predicted 405-amino acid protein homologous to vertebrate and invertebrate TnT and includes an invertebrate-specific COOH-terminal tail. The mup-2 mutations lie within these cDNA sequences: mup-2(up1) is a termination codon near NH2 terminus (Glu94) and mup-2(e2346ts) is a termination codon in the COOH-terminal invertebrate-specific tail (Trp342). TnT is a muscle contractile protein that, in association with the thin filam...Continue Reading

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Citations

Feb 9, 2002·Molecular Reproduction and Development·Yhong-Hee ShimYoung-Ki Paik
Jan 20, 2011·Journal of Cell Science·Maria I FerranteDerek L Stemple
Sep 6, 2014·Journal of Genetics·Salam Herojeet SinghUpendra Nongthomba
Oct 27, 2011·Methods : a Companion to Methods in Enzymology·Takashi Obinata, Naruki Sato
May 6, 2008·Biochimica Et Biophysica Acta·Hiroyuki TanakaTakao Ojima
May 4, 2007·Experimental Cell Research·Xiaojian XuMyeongwoo Lee
Mar 4, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Razia RuksanaHiroaki Kagawa
May 23, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chung-Der HsiaoHuai-Jen Tsai
Feb 28, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kanako OnoShoichiro Ono
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shoichiro Ono
Apr 6, 2016·Developmental Biology·Alyssa D CecchetelliErin J Cram
Oct 23, 2010·Archives of Biochemistry and Biophysics·Bin Wei, J-P Jin
Aug 23, 2000·American Journal of Human Genetics·J J JohnstonL G Biesecker
Jul 19, 2014·International Journal for Parasitology·Martina OndrovicsAnja Joachim
May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E J Hubbard, D Greenstein
Dec 3, 2016·ELife·Thibaut BrunetDetlev Arendt
Nov 28, 2018·Journal of Cell Science·Nicole GreenErika R Geisbrecht
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma

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