Developmental, hormonal, and nutritional regulation of porcine adipose triglyceride lipase (ATGL)

Lipids
Jeffrey A DeiuliisKichoon Lee

Abstract

Adipose triglyceride lipase (ATGL) is a newly identified lipase. We report for the first time the porcine ATGL sequence and characterize ATGL gene and protein expression in vitro and in vivo. Adult pig tissue expresses ATGL at high levels in the white adipose and muscle tissue relative to other tested tissues. We show that within the white adipose tissue ATGL is expressed at higher levels in the adipocyte than in the stromal-vascular fraction. Additionally, ATGL expression increases dramatically in the subcutaneous adipose during adipose development and maturation, as well as during in vitro adipogenesis. Peroxisome proliferator-activated receptor gamma transcript levels increased concomitant with ATGL gene expression, suggesting a possible role in the regulation of ATGL by adipogenic regulators. In vitro treatment of differentiated primary pig preadipocytes with insulin and forskolin decreased ATGL gene expression in a dose-dependent manner, suggesting ATGL transcript levels are hormone sensitive. In vivo experimentation showed that calorie-restriction in gilts resulted in increased ATGL mRNA and protein levels in subcutaneous and peri-renal fat tissues. Our data demonstrate that ATGL expression reacts to hormonal stimuli and ...Continue Reading

References

Aug 1, 1986·Journal of Animal Science·G J Hausman, R G Kauffman
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R L McNeelH J Mersmann
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 9, 2002·Molecular and Cellular Biology·Yang Soo MoonHei Sook Sul
Feb 18, 2003·The Journal of Clinical Investigation·Kichoon LeeHei Sook Sul
Jan 1, 1963·The British Journal of Nutrition·M J MANNERS, M R MCCREA
Apr 8, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Basem M AbdallahMoustapha Kassem
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner
Jul 13, 2005·Molecular and Cellular Endocrinology·Susan KralischMathias Fasshauer
Aug 2, 2005·Cell Metabolism·Sebastian GrönkeRonald P Kühnlein
Sep 30, 2005·International Journal of Experimental Pathology·James R TurkM Harold Laughlin
Oct 22, 2005·EMBO Reports·Elena SmirnovaCatherine L Jackson
Oct 27, 2005·Diabetes·Dominique LanginPeter Arner
Oct 28, 2005·The Journal of Nutrition·Werner G Bergen, Harry J Mersmann
Aug 12, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jeffrey A DeiuliisKichoon Lee
Oct 26, 2006·Obesity·Christelle Guillerm-RegostFlorence Gondret
Sep 13, 2007·American Journal of Physiology. Endocrinology and Metabolism·Erin E KershawJeffrey S Flier

❮ Previous
Next ❯

Citations

May 11, 2010·Journal of Applied Genetics·Q-H NieX-Q Zhang
May 11, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·P OliverA Palou
May 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Johanna SamulinHilde Sundvold
Apr 27, 2011·Journal of Cellular Physiology·Paola VigilanzaMaria Rosa Ciriolo
May 2, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jian SunLi-Qiao Chen
Jul 25, 2009·Molecular and Cellular Biochemistry·Yu-cheng LiGong-she Yang
Aug 6, 2008·Molecular and Cellular Biochemistry·Qinghua NieXiquan Zhang
Jul 17, 2013·Experimental Biology and Medicine·Michael V DodsonGary J Hausman
Jan 22, 2010·Molecular Biology Reports·Qinghua NieXiquan Zhang
Aug 31, 2010·Journal of Animal Science·T ShanY Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.