Developmental mechanisms of migratory muscle precursors in medaka pectoral fin formation

Development Genes and Evolution
Saori Tani-MatsuhanaKunio Inoue

Abstract

Limb muscles are formed from migratory muscle precursor cells (MMPs) that delaminate from the ventral region of dermomyotomes and migrate into the limb bud. MMPs remain undifferentiated during migration, commencing differentiation into skeletal muscle after arrival in the limb. However, it is still unclear whether the developmental mechanisms of MMPs are conserved in teleost fishes. Here, we investigate the development of pectoral fin muscles in the teleost medaka Oryzias latipes. Expression of the MMP marker lbx1 is first observed in several somites prior to the appearance of fin buds. lbx1-positive cells subsequently move anteriorly and localize in the prospective fin bud region to differentiate into skeletal muscle cells. To address the developmental mechanisms underlying fin muscle formation, we knocked down tbx5, a gene that is required for fin bud formation. tbx5 morphants showed loss of fin buds, whereas lbx1 expression initiated normally in anterior somites. Unlike in normal embryos, expression of lbx1 was not maintained in migrating fin MMPs or within the fin buds. We suggest that fin MMPs appear to undergo two phases in their development, with an initial specification of MMPs occurring independent of fin buds and a se...Continue Reading

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Citations

Aug 7, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathleen A SagarinStephen H Devoto
Apr 27, 2019·Development·Jared C TalbotSharon L Amacher

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Methods Mentioned

BETA
delamination
PCR
Confocal microscopy

Software Mentioned

Medaka Ensemble
Ensemble

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