Nov 7, 1994

Developmental modulation of blood-brain-barrier glucose transport in the rabbit

Brain Research
E M CornfordE M Landaw

Abstract

Blood-brain barrier (BBB) glucose transport rates were measured using the intracarotid injection method in newborn, 14-day-old suckling, 28-day-old weanling and adult rabbits, and compared with membrane transporter density. Light microscope immunochemistry confirmed the presence of the GLUT1 glucose transporter isoform in these rabbits. Quantitative electron microscopic immunogold analyses of GLUT1-immunoreactive sites per micrometer of capillary membrane indicated GLUT1 density increased with age, and correlated with in vivo measurements of Vmax. Maximal transport velocities (Vmax) of glucose transfer (an indicator of the activity and relative number of transporter proteins) increased significantly (P = 0.05) with age: in neonates Vmax = 0.61 mumol.min-1.g-1, in sucklings Vmax = 0.68 mumol.min-1.g-1, in weanlings Vmax = 0.88 mumol.min-1.g-1, and in adults Vmax = 1.01 mumol.min-1 g-1. Cerebral blood flow (CBF) rates, increased with age from 0.19 and 0.26 ml.min-1.g-1 in neonates and sucklings to 0.51 (weanlings) and 0.70 (adults) ml.min-1.g-1. Non-linear regression analyses indicated the half-saturation constant (Km) for glucose transport ranged from 13 mM in adult rabbits to 19 mM in 14-day-old sucklings: differences in Km wer...Continue Reading

  • References35
  • Citations9

References

  • References35
  • Citations9

Citations

Mentioned in this Paper

Cerebral Blood Flow Imaging
Blood - Brain Barrier Anatomy
Brain
Immunocytochemistry
Carbon Radioisotopes
Glucose, (beta-D)-Isomer
Senility
SLC2A1 gene
Glucose Import
Chinchilla Rabbits

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