Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy

Frontiers in Plant Science
Dominik NovákJozef Samaj

Abstract

The development of the root apex is determined by progress of cells from the meristematic region to the successive post-mitotic developmental zones for transition, cell elongation and final cell differentiation. We addressed root development, tissue architecture and root developmental zonation by means of light-sheet microscopic imaging of Arabidopsis thaliana seedlings expressing END BINDING protein 1c (EB1c) fused to green fluorescent protein (GFP) under control of native EB1c promoter. Unlike the other two members of the EB1 family, plant-specific EB1c shows prominent nuclear localization in non-dividing cells in all developmental zones of the root apex. The nuclear localization of EB1c was previously mentioned solely in meristematic cells, but not further addressed. With the help of advanced light-sheet microscopy, we report quantitative evaluations of developmentally-regulated nuclear levels of the EB1c protein tagged with GFP relatively to the nuclear size in diverse root tissues (epidermis, cortex, and endodermis) and root developmental zones (meristem, transition, and elongation zones). Our results demonstrate a high potential of light-sheet microscopy for 4D live imaging of fluorescently-labeled nuclei in complex sampl...Continue Reading

References

Mar 6, 1999·Current Opinion in Plant Biology·B Scheres, T Berleth
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Aug 10, 2000·Molecular Biology of the Cell·E ChytilovaD W Galbraith
Feb 15, 2002·Nature·Detlef Weigel, Gerd Jürgens
Dec 12, 2002·Molecular Biology of the Cell·Jennifer S TirnauerTimothy J Mitchison
Oct 15, 2003·Nature Cell Biology·Jordi ChanClive W Lloyd
Jul 22, 2004·Plant Physiology·Jozef SamajDiedrik Menzel
Aug 18, 2004·Protein Engineering, Design & Selection : PEDS·Tanja la CourSøren Brunak
Dec 14, 2004·Plant Physiology·Sherryl R BisgroveDarryl L Kropf
Dec 1, 2005·Journal of Experimental Botany·A Glyn BengoughTracy A Valentine
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Feb 3, 2007·BioTechniques·Michael R LamprechtAnne E Carpenter
Feb 8, 2007·Journal of Plant Research·Takahiro Hamada
Mar 17, 2007·Current Biology : CB·Raffaele Dello IoioSabrina Sabatini
Mar 7, 2008·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Sep 23, 2008·Plant Molecular Biology·Eva Benková, Jan Hejátko
Jan 21, 2009·Cell Research·Jian RenXuebiao Yao
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kamil RuzickaEva Benková
Mar 4, 2009·The Journal of Cell Biology·Yulia KomarovaAnna Akhmanova
Jan 14, 2010·Journal of Cell Science·Shinichiro KomakiTakashi Hashimoto
Jul 16, 2010·The Plant Journal : for Cell and Molecular Biology·Eric S McLamoreD Marshall Porterfield
Sep 8, 2010·Current Opinion in Cell Biology·Kai Jiang, Anna Akhmanova
May 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sumiko AdachiMasaaki Umeda
Jun 30, 2011·The Plant Journal : for Cell and Molecular Biology·Alexis MaizelErnst H K Stelzer
Mar 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jalean J PetrickaPhilip N Benfey
Jan 8, 2013·Trends in Plant Science·Olga ŠamajováJozef Šamaj
Feb 27, 2013·The New Phytologist·Anna DoskočilováPavla Binarová
Oct 10, 2013·Frontiers in Plant Science·František Baluška, Stefano Mancuso

❮ Previous
Next ❯

Citations

Mar 1, 2018·Annual Review of Plant Biology·George KomisJozef Šamaj
Mar 3, 2020·Annual Review of Plant Biology·Natalie M ClarkRosangela Sozzani
Sep 7, 2018·Nature Plants·Miroslav OvečkaJozef Šamaj

❮ Previous
Next ❯

Datasets Mentioned

BETA
AT5G62500
AT5G67270

Methods Mentioned

BETA
transgenic

Software Mentioned

CellProfiler
DOG
NetNES
Zen
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.