PMID: 11335004May 4, 2001Paper

Developmental patterns of NF-kappaB activation during acute hypoxia in the caudal brainstem of the rat

Brain Research. Developmental Brain Research
N SimakajornboonD Gozal

Abstract

NF-kappaB, an ubiquitous transcription factor which plays a major role in the regulation of stress-related genes, is activated during environmental hypoxia in the dorsocaudal brainstem of adult rats. To examine the developmental pattern of NF-kappaB basal activity in the brainstem and the response to hypoxia, electromobility shift assays and immunohistochemical staining for the P65 subunit of NF-kappaB were performed in caudal brainstem samples of rats at 2, 5, 10, 15, and 60 days postnatal age, following normoxic or hypoxic (1 h in 10% O2) exposures. In addition, the expression of IkappaB-alpha, and IkappaB kinases (ikk)-alpha and -beta was also examined using Western blots. Basal NF-kappaB nuclear activity and nuclear P65 immunoreactivity increased with maturation. In contrast, hypoxia induced enhanced activation of NF-kappaB and nuclear translocation of P65 in youngest animals. Expression of both IkappaB-alpha and ikk-alpha was highest in the more immature rats, and decreased with postnatal age. In contrast, ikk-beta expression was unchanged over time. We conclude that NF-kappaB activity in caudal brainstem is developmentally regulated, and that hypoxia-induced NF-kappaB activation is more prominent in youngest rats. We post...Continue Reading

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Citations

Jun 14, 2005·Respiratory Physiology & Neurobiology·Narong Simakajornboon, Teeradej Kuptanon
Jun 25, 2003·Biochemical and Biophysical Research Communications·Hyun-Ja JeongHyung-Min Kim
Jul 26, 2002·Pediatrics International : Official Journal of the Japan Pediatric Society·Jolanta M DzikElzbieta Walajtys-Rode
Jan 27, 2007·American Journal of Respiratory and Critical Care Medicine·Cristina M AlviraMarlene Rabinovitch
Sep 3, 2004·The Journal of Clinical Investigation·Guang YangPhyllis A Dennery
Nov 10, 2011·Cellular and Molecular Life Sciences : CMLS·Ulrike BruningAlex Cheong
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Myriam BernaudinPascale Schumann-Bard
Jan 6, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yong-Sun LeePak H Chan
Sep 26, 2006·Biochemical Pharmacology·Sylvie Mémet
Mar 16, 2006·Current Opinion in Neurology·Ying-Chao Chang, Chao-Ching Huang

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