Developmental plasticity in reptiles: Insights from temperature-dependent gene expression in wall lizard embryos

Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
Nathalie FeinerTobias Uller

Abstract

Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20% less total RNA than those incubated at benign temperatures, presumably reflecting lower rates of transcription at cool temperature. After normalizing for differences in total amounts of input RNA, we find that approximately 50% of all transcripts show significant expression differences between the two incubation temperatures. Transcripts with the most extreme changes in expression profiles are associated with transcriptional and translational regulation and chromatin remodeling, suggesting possible...Continue Reading

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Citations

Jun 30, 2018·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Daniel A WarnerArthur Georges
May 29, 2018·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Geoffrey M WhileLisa E Schwanz
Feb 23, 2020·Frontiers in Physiology·Sunil Kumar SinghTurk Rhen
May 30, 2020·Integrative and Comparative Biology·Beth A McCawLesley T Lancaster
Apr 14, 2021·Evolution & Development·Thomas J Sanger

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