PMID: 7528550Sep 1, 1994Paper

Developmental potential of rhesus monkey embryos produced by in vitro fertilization

Biology of Reproduction
L ZhangD P Wolf

Abstract

This study was an examination of the developmental potential of in vitro fertilization (IVF)-produced rhesus monkey embryos that were cultured in medium alone or cocultured with various cell types. End points were the quality and yield of embryos attaining the expanded or hatched blastocyst stage. A total of 96 IVF-produced embryos were cryopreserved and thawed, and 90 embryos were considered intact and suitable for culture. These embryos were placed into one of five treatment groups consisting of four different cell supports and medium alone. Two primary cultures (bovine oviductal cells [bOVID] and bovine cumulus cells [bCUM]) and two established cell lines (Vero cells and buffalo rat liver cells [BRL]) were utilized for coculture of embryos. Embryos were cultured for up to 14 days, and growth curves were established for all embryos that expanded and/or hatched. The developmental rate for embryos classified as viable varied substantially; in number of days to reach a given stage, early morulae ranged from Days 3 to 9 post-insemination, morulae from Days 4 to 9, blastocysts from Days 6 to 11, expanded blastocysts from Days 7 to 12, and hatched blastocysts from Days 9 to 15. On the basis of developmental curves, 30% of the embry...Continue Reading

Citations

May 1, 1996·Molecular Reproduction and Development·A M Weston, D P Wolf
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qiang SunJoe Z Tsien
Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Don P Wolf
Jan 1, 2010·The International Journal of Developmental Biology·Michelle L SparmanShoukhrat M Mitalipov
Dec 4, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Nobuhiro ShimozawaTadashi Sankai
Jun 8, 2011·Fertility and Sterility·Catherine A VandevoortDennis R Stewart
Jan 23, 1999·Biology of Reproduction·D P WolfM Zelinski-Wooten
Jan 5, 2001·Human Reproduction·K D NusserD P Wolf
Aug 31, 2001·Human Reproduction·R R YeomanD P Wolf
Jul 12, 2005·Human Reproduction·Allen C EndersGordon C Douglas

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