PMID: 9192638Jun 24, 1997Paper

Developmental regulation and the role of insulin and insulin receptor in metanephrogenesis

Proceedings of the National Academy of Sciences of the United States of America
Z Z LiuY S Kanwar

Abstract

The insulin family of peptides and their receptors influence cellular growth in very early preimplantation embryos. In this study their expression and role in renal organogenesis was investigated. By immunofluorescence microscopy and in situ hybridization, insulin receptor (IR) expression was seen in the ureteric bud branches and early nephron precursors in mouse metanephroi harvested at day 13 of gestation. The expression gradually decreased in successive stages of gestation, and it was confined mainly to renal tubules in 1-week-old mice. Similar developmental regulation of the IR and insulin was observed by reverse transcriptase-polymerase chain reaction (RT-PCR) analyses. Addition of insulin into the culture medium at low concentrations, ranging from 40 to 400 ng/ml, induced trophic changes and increased [3H]thymidine incorporation in the embryonic renal explants, and inclusion of IR beta-subunit-specific antisense oligodeoxynucleotide caused marked dysmorphogenesis and growth retardation of the metanephroi. Specificity of the antisense effect was reflected by immunoprecipitation experiments in which translational blockade of the beta subunit of the IR was observed. RT-PCR analyses revealed that the alpha subunit of the IR w...Continue Reading

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Citations

Mar 22, 2003·Current Urology Reports·Chong Min Kim, Kenneth I Glassberg
Apr 5, 2003·Kidney International·Yashpal S KanwarJon W Lomasney
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D L SokolA M Gewirtz
Jan 27, 1998·Biochemical and Molecular Medicine·N J WebsterB L Seely
Sep 19, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K JohkuraA Nakazawa
Oct 3, 1999·Physiological Reviews·M F HorsterS M Huber
Jan 7, 2004·American Journal of Physiology. Renal Physiology·Yashpal S KanwarJon W Lomasney
Oct 1, 1998·The American Journal of Physiology·E I WallnerY S Kanwar
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Yashpal S KanwarSatish K Srivastava
Nov 13, 2018·The Journal of Clinical Investigation·Matthew J MurthaJohn David Spencer
Mar 6, 2021·The Journal of Biological Chemistry·Adrian Kee Keong TeoRohit N Kulkarni
Feb 8, 2021·Diabetes Research and Clinical Practice·Zain AwamlehRosanna Weksberg
Oct 15, 2018·Molecular Metabolism·Manoj K GuptaRohit N Kulkarni
Mar 19, 1999·Methods : a Companion to Methods in Enzymology·D L Sokol, A M Gewirtz

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