PMID: 9443075Jan 27, 1998Paper

Developmental regulation of 14-3-3 epsilon isoform in rat heart

Journal of Cellular Biochemistry
S C LukM M Waye

Abstract

Human heart cDNA sequencing yielded a cDNA clone that is similar in DNA and amino acid sequences to that of mouse 14-3-3 epsilon isoform. The 6xHis-tagged H1433 epsilon recombinant protein was expressed in Escherichia coli and its size was approximately 30 kDa. From Northern blot results with human multiple tissues, human skeletal muscle was found to have the highest level of h1433 epsilon mRNA expression, whereas Northern blots of human cancer cell lines detected the highest mRNA level of h1433 epsilon in colorectal adenocarcinoma SW480. The protein expression level of h1433 epsilon and Raf-1 is found to be regulated coordinately during rat heart development, and their protein expression was highest from 14.5 to 16.5 days postcoitum.

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G LuR J Ferl
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T IchimuraY Takahashi
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S ConklinD Beach
Jun 1, 1996·Digestive Diseases and Sciences·S EggsteinE H Farthmann

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Citations

Feb 25, 2003·Journal of Experimental Botany·Simone de F MaraschinMei Wang
Apr 20, 2006·Clinical and Experimental Pharmacology & Physiology·Ming HeZhijun Luo
Oct 17, 2012·Molecular and Cellular Biology·Yasuhiro KosakaLuca Brunelli
Feb 7, 2001·Biochemical and Biophysical Research Communications·H C ChanM M Waye

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