Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.

Current Biology : CB
Esther K KiesermanJohn B Wallingford

Abstract

Mitosis and cytokinesis not only ensure the proper segregation of genetic information but also contribute importantly to morphogenesis in embryos. Cytokinesis is controlled by the central spindle, a microtubule-based structure containing numerous microtubule motors and microtubule-binding proteins, including PRC1. We show here that central spindle assembly and function differ dramatically between two related populations of epithelial cells in developing vertebrate embryos examined in vivo. Compared to epidermal cells, early neural epithelial cells undergo exaggerated anaphase chromosome separation, rapid furrowing, and a marked reduction of microtubule density in the spindle midzone. Cytokinesis in normal early neural epithelial cells thus resembles that in cultured vertebrate cells experimentally depleted of PRC1. We find that PRC1 mRNA and protein expression is surprisingly dynamic in early vertebrate embryos and that neural-plate cells contain less PRC1 than do epidermal cells. Expression of excess PRC1 ameliorates both the exaggerated anaphase and reduced midzone microtubule density observed in early neural epithelial cells. These PRC1-mediated modifications to the cytokinetic mechanism may be related to the specialization ...Continue Reading

References

Jul 1, 1975·The American Journal of Anatomy·R Bellairs, M Bancroft
Apr 1, 1991·Neuron·W A Harris, V Hartenstein
Aug 1, 1987·The Journal of Cell Biology·W M Saxton, J R McIntosh
Mar 23, 2000·Mechanisms of Development·L S Gammill, H Sive
Jan 11, 2001·Developmental Biology·Y Saka, J C Smith
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M Glotzer
Jun 26, 2002·The Journal of Cell Biology·Cristiana MollinariRobert L Margolis
Mar 1, 1965·The Journal of Cell Biology·A KRISHAN, R C BUCK
Oct 1, 1961·The Journal of Experimental Zoology·R RAPPAPORT
Sep 3, 2004·Current Biology : CB·Fiammetta VernìMaurizio Gatti
Dec 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Changjun Zhu, Wei Jiang
Apr 5, 2005·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·John B Wallingford
Apr 14, 2006·Nature·Tamara A PotapovaGary J Gorbsky

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Citations

Jun 28, 2012·Biomechanics and Modeling in Mechanobiology·G Wayne Brodland, Jim H Veldhuis
Dec 9, 2008·Nature Cell Biology·Ann L Miller, William M Bement
Oct 20, 2011·Nature Communications·Andreas W EttingerWieland B Huttner
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Michael Glotzer
Feb 10, 2016·Developmental Cell·Joao FirminoJerome Gros
Mar 17, 2009·Trends in Cell Biology·George von Dassow
Apr 1, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marie Delattre, Marie-Anne Félix
Jan 20, 2011·Cytoskeleton·Katherine R SmithJohn B Wallingford
Jan 15, 2016·Molecular Biology of the Cell·Ryota UeharaGohta Goshima
Jul 26, 2019·Biological Reviews of the Cambridge Philosophical Society·Zhen-Yu SheMing-Hui Lu
Apr 15, 2017·Frontiers in Oncology·Søs Grønbæk MathiassenFrancesco Cecconi
May 5, 2010·Cold Spring Harbor Protocols·John B Wallingford
May 5, 2010·Cold Spring Harbor Protocols·Esther K KiesermanJohn B Wallingford

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