PMID: 2107027Mar 9, 1990Paper

Developmental regulation of IgM secretion: the role of the carboxy-terminal cysteine

Cell
Roberto SitiaC Milstein

Abstract

B lymphocytes do not secrete IgM, and plasma cells only secrete IgM polymers. Here we show that both events are attributable to the tailpiece found at the carboxyl terminus of mus chains, and we specifically implicate Cys-575. Thus, if Cys-575 was mutated, IgM was secreted by B cells. Similarly, a mutant IgG containing a mus tailpiece became largely retained within the cell; secretion was restored upon mutation of the tailpiece cysteine. Removal of Cys-575 also allowed hypersecretion of monomeric IgM by plasmacytoma cells. Following further removal of Cmu1, heavy chains were secreted in the absence of light chains. Thus, in B and plasma cells, Cys-575 is involved both in the polymerization of IgM and in intracellular retention of unpolymerized intermediates.

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Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P S Reddy, R B Corley
Jan 1, 1993·Cytotechnology·A RubartelliR Sitia
Jun 1, 1994·Molecular Immunology·W R Brown, J E Butler
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Jul 1, 1994·Bio/technology·R I Smith, S L Morrison
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ajit-Singh Dhaunchak, Klaus-Armin Nave
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eelco van AnkenIneke Braakman
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T D RandallR B Corley
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