Developmental regulation of insulin receptor gene in sciatic nerves and role of insulin on glycoprotein P0 in the Schwann cells

Peptides
Abhijith Shettar, Gopal Muttagi

Abstract

In view of the observations that Schwann cells contain insulin receptors, in the present study, we have investigated the developmental regulation of insulin receptor gene in the sciatic nerves of different postnatal age group rats. We have also investigated the role of insulin in the expression of the major PNS myelin glycoprotein P zero (P0) in normal as well as high glucose conditions in primary rat Schwann cells. The expression of insulin receptor gene in sciatic nerves appeared to be differentially regulated. The steady-state levels of insulin receptor mRNA increased remarkably during development and after postnatal day 10, when the peak of myelin structural gene (P0) expression occur and slowly increased further until at least postnatal day 90 in parallel with the growth of the myelin sheath. By employing immunofluorescence and RT-PCR, we observed significant increase in the P0 protein and mRNA levels in Schwann cells in response to the insulin than in insulin deprived counterparts. The presence of insulin in the high glucose medium ameliorated the altered protein and mRNA of P0 in Schwann cells compared to the insulin deprived counterparts. These studies demonstrate the importance of insulin and its receptor as possible r...Continue Reading

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Citations

Jun 4, 2013·Trends in Neurosciences·Jennifer ZenkerRoman Chrast
Sep 14, 2013·Journal of the Peripheral Nervous System : JPNS·Caleb W GroteDouglas E Wright
Jan 31, 2017·Nature Reviews. Neurology·Nádia P GonçalvesTroels S Jensen
Jan 10, 2017·Frontiers in Neuroscience·Caleb W Grote, Douglas E Wright
Apr 27, 2019·Frontiers in Cellular Neuroscience·Arul DuraikannuDouglas W Zochodne
Mar 7, 2021·Biomolecules·Jocelyn M BretonDaniela Kaufer
Apr 18, 2018·Molecular and Cellular Biology·Wang-Yang XuZhu-Gang Wang
Jul 24, 2021·Molecular Neurobiology·Pariya KhodabakhshLeila Dargahi
Nov 14, 2021·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Atul Rawat, Brett M Morrison

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