Developmental regulation of Mac25/insulin-like growth factor-binding protein-7 expression in skeletal myogenesis

Experimental Cell Research
S E DamonL S Quinn

Abstract

Mac25 is a newly discovered member of the insulin-like growth factor-binding protein (IGFBP) family, recently assigned the name IGFBP-7, Mac25/IGFBP-7 is hypothesized to have growth-suppressing activity, since mac25/IGFBP-7 mRNA is down-regulated in several tumor cell lines and is highly expressed in senescent mammary epithelial cells. In this study, mac25/ IGFBP-7 mRNA expression was characterized in the C2 skeletal myogenic cell line, which undergoes a transition from actively dividing, undifferentiated myoblasts to nondividing, differentiated myotubes. Mac25/ IGFBP-7 mRNA levels were 2.5-fold higher in dividing C2 myoblasts than in nondividing myotubes. The inverse correlation between mac25/IGFBP-7 expression and myogenic differentiation was further examined by treating myogenic cultures with transforming growth factor-beta (TGF-beta) or insulin-like growth factor-I (IGF-I). TGF-beta inhibited myogenic differentiation by 98% and stimulated mac25/IGFBP-7 mRNA expression 2-fold. IGF-I stimulated differentiation by 50% and inhibited mac25/IGFBP-7 expression 2- to 3-fold. These findings indicate that, in contrast to other cell systems examined so far, expression of this new member of the IGFBP family is not always correlated wit...Continue Reading

Citations

May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A DegeorgesR A Sikes
Dec 22, 1999·Endocrine Reviews·V HwaR G Rosenfeld
Oct 21, 1999·Molecular Genetics and Metabolism·L A WetterauP Cohen
Aug 15, 1998·Molecular and Cellular Endocrinology·C Collet, J Candy

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