Developmental regulation of mitogen-activated protein kinase-activated kinases-2 and -3 (MAPKAPK-2/-3) in vivo during corpus luteum formation in the rat

Molecular Endocrinology
E T MaizelsM Hunzicker-Dunn

Abstract

The current study investigates the activation in vivo and regulation of the expression of components of the p38 mitogen-activated protein kinase (MAPK) pathway during gonadotropin-induced formation and development of the rat corpus luteum, employing a sequential PMSG/human CG (hCG) treatment paradigm. We postulated that the p38 MAPK pathway could serve to promote phosphorylation of key substrates during luteal maturation, since maturing luteal cells, thought to be cAMP-nonresponsive, nevertheless maintain critical phosphoproteins. Both p38 MAPK and its upstream activator MAPK kinase-6 (MKK6) were found to be chronically activated during the luteal maturation phase, with activation detected by 24 h post hCG and maintained through 4 days post hCG. The p38 MAPK downstream protein kinase target termed MAPK-activated protein kinase-3 (MAPKAPK-3) was newly induced at both mRNA and protein levels during luteal formation and maturation, while mRNA and protein expression of the closely related MAPKAPK-2 diminished. Two potential substrates for MAPKAPKs, the small heat shock protein HSP-27 and the cAMP regulatory element binding protein CREB, were monitored in vivo for phosphorylation. HSP-27 phosphorylation was not modulated during lute...Continue Reading

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Citations

Mar 23, 2002·Molecular and Cellular Biology·Giselle R WigginJ Simon C Arthur
May 23, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Thomas AndrieuHervé Mittre
Feb 12, 2009·Gastroenterology·Hironobu TsukadaKazuaki Chayama
Jun 16, 2005·The Journal of Investigative Dermatology·Arianna L KimDavid R Bickers
Aug 11, 2004·Experimental Cell Research·Vera ZakowskiStephan Ludwig
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Nov 30, 2018·Endocrine Reviews·Diane M DuffyThomas E Curry
Jan 24, 2007·Human Reproduction Update·Darryl L Russell, Rebecca L Robker
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Sep 1, 2004·Cellular Signalling·Mona JohannessenUgo Moens
Aug 30, 2005·Cellular Signalling·Marit Pedersen DelghandiUgo Moens

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