PMID: 9164858May 15, 1997Paper

Developmental regulation of mRNA species for types II, IX and XI collagens during mouse embryogenesis

The Biochemical Journal
M PeräläE Vuorio

Abstract

Several techniques were used to study the co-ordination of mRNA levels for five constituent chains of cartilage collagen fibrils during mouse development. Short cDNA clones were first constructed for mouse and human alpha3(IX) and for mouse proalpha1(XI) collagen mRNA species. Northern analysis of developing mouse embryos revealed that the mRNA species for alpha1, alpha2 and alpha3 chains of type IX collagen peaked earlier than those for proalpha1(II) and proalpha1(XI) collagen chains. Quantification of these mRNA species by slot-blot hybridization confirmed this developmental regulation: the mRNA ratios for type II/type IX/type XI collagens changed from 5.7:1:0.6 (at embryonic day 12.5) to 10.6:1:0.9 (in newborn mice). However, the genes coding for the three chains of type IX collagen seemed to be under more co-ordinated regulation during mouse development. In addition to high mRNA levels in cartilages and the eye, low levels of type IX collagen transcripts were identified in brain and skin of newborn mouse using RNase protection and reverse transcriptase-PCR assays. Finally, hybridization in situ revealed identical tissue distributions of the three type IX collagen mRNA species during early chondrogenesis but somewhat more wi...Continue Reading

Citations

Sep 11, 2012·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·D KrugJ Kramer
Feb 7, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chiachien Jake WangIchiro Nishimura
Sep 24, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Andreas TagarielloBernhard Zabel
Aug 29, 2012·Journal of Oncology·Roberta MalaguarneraAntonino Belfiore
Mar 29, 2001·Experimental Eye Research·T IhanamäkiE Vuorio
Jan 28, 2006·Endocrinology·Maija P ValtaPirkko L Härkönen
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·M S ThompsonG N Duda
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C G BönnemannL M Kunkel
Nov 5, 2002·Journal of Cell Science·Claudia HegertJürgen Rohwedel

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