PMID: 6165022Mar 1, 1981Paper

Developmental regulation of myelin basic protein in dispersed cultures

Proceedings of the National Academy of Sciences of the United States of America
E Barbarese, S E Pfeiffer

Abstract

The expression of myelin basic protein, a major component of the myelin membrane, was studied in the absence of myelin formation in a unique situation in which these two processes have been uncoupled. The oligodendrocytes that contained myelin basic protein were identified by immunofluorescence in primary dispersed cultures derived from 20-day-old fetal rat brain. Their number increased 20-fold between 15 and 34 days in culture. Morphologically identifiable myelin was never observed. The oligodendrocytes elaborated a complex network of processes and membranous sheets resembling "unfurled" myelin. Myelin basic protein appeared concomitantly in the perikaryon, processes, and membranous sheets of the oligodendrocytes and remained distributed in these compartments throughout the culture period. The oligodendrocytes synthesized the four forms of myelin basic protein found in rodent brain with molecular weights of 21,500, 18,500, 17,000, and 14,000 and modulated their expression with time in culture. The onset of rapid myelin basic protein accumulation, as measured by radioimmunoassay, took place after 25 days in culture. Myelin basic protein accumulated at the rate of 0.2 fmol per oligodendrocyte per day and reached a level of 300 p...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E BarbareseJ H Carson
Oct 1, 1978·Journal of Neurochemistry·E BarbareseP E Braun
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerH D Webster
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Jul 5, 1974·Brain Research·C A Fischer, P Morell

❮ Previous
Next ❯

Citations

Jun 1, 1996·Journal of Neuroscience Research·B Q KreiderD Pleasure
Jul 15, 1996·Journal of Neuroscience Research·C Richter-Landsberg, M Heinrich
Dec 24, 1997·Journal of Neuroscience Research·D GayA Bhandoola
Nov 1, 1990·Journal of Neuroscience Research·U VielkindM C Bohn
Dec 1, 1990·Journal of Neuroscience Research·S U Kim
Jan 1, 1995·Journal of Neuroscience Research·B Q KreiderD Pleasure
Nov 1, 1995·Journal of Neuroscience Research·L ZhouR H Miller
Dec 1, 1982·Journal of Neurocytology·N K GonatasD H Silberberg
Jan 1, 1984·Medical Microbiology and Immunology·C F Beranek, N Herschkowitz
Jan 1, 1988·Molecular Neurobiology·A T Campagnoni, W B Macklin
Nov 26, 1990·Brain Research·D KajikawaM Shimo-oku
Apr 1, 1983·Developmental Biology·J H CarsonE Barbarese
Jun 1, 1989·Journal of the Neurological Sciences·E Barbarese, C Barry
Mar 1, 1992·Journal of the Neurological Sciences·J McLaurin, M A Moscarello
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J F PodusloP J Dyck
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F AloisiG Levi
Feb 1, 1986·The Journal of Cell Biology·M Dubois-DalcqR A Lazzarini
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R P Saneto, J de Vellis
Aug 1, 1994·Neurochemical Research·Q Q Mu, C Dyer
Mar 29, 2001·Microscopy Research and Technique·G AlmazánC Guaza
Oct 13, 2006·Brain, Behavior, and Immunity·Andrew W ZimmermanDavid A Pearce
Oct 1, 1987·Journal of Neurochemistry·E N BenvenisteJ N Whitaker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.