Developmental restriction of embryonic calvarial cell populations as characterized by their in vitro potential for chondrogenic differentiation

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
C D TomaL C Gerstenfeld

Abstract

The mechanism(s) by which the cells within the calvaria tissue are restricted into the osteogenic versus the chondrogenic lineage during intramembranous bone formation were examined. Cells were obtained from 12-day chicken embryo calvariae after tissue condensation, but before extensive osteogenic differentiation, and from 17-day embryo calvariae when osteogenesis is well progressed. Only cell populations from the younger embryos showed chondrogenic differentiation as characterized by the expression of collagen type II. The chondrocytes underwent a temporal progression of maturation and endochondral development, demonstrated by the expression of collagen type II B transcript and expression of collagen type X mRNA. Cell populations from both ages of embryos showed progressive osteogenic differentiation, based on the expression of osteopontin, bone sialoprotein, and osteocalcin mRNAs. Analysis using lineage markers for either chondrocytes or osteoblasts demonstrated that when the younger embryonic cultures were grown in conditions that were permissive for chondrogenesis, the number of chondrogenic cells increased from approximately 15 to approximately 50% of the population, while the number of osteogenic cells remained almost con...Continue Reading

References

Sep 1, 1978·Developmental Biology·P G ConstantinidesP A Jones
Nov 1, 1979·Developmental Biology·P Osdoby, A I Caplan
Jan 1, 1975·The Anatomical Record·B K Hall, H N Jacobson
Nov 1, 1975·Clinical Orthopaedics and Related Research·Y Terashima, M R Urist
Mar 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A A ColeT M Schmid
Sep 1, 1991·Calcified Tissue International·A Yamaguchi, A J Kahn
Feb 1, 1985·The Journal of Cell Biology·T M Schmid, T F Linsenmayer
Nov 1, 1989·The Journal of Cell Biology·P BrucknerM A Spycher
Jul 1, 1987·Developmental Biology·L C GerstenfeldJ B Lian
Jun 1, 1985·Molecular and Cellular Biology·M H FinerH Boedtker
Mar 1, 1974·The Journal of Experimental Zoology·M Solursh, S Meier
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
May 25, 1984·Nucleic Acids Research·M F YoungM E Sobel
Sep 4, 1984·Journal of Immunological Methods·H Towbin, J Gordon
Jul 1, 1982·Journal of Cellular Physiology·H A KodamaS Kasai
Jan 29, 1980·Biochemical and Biophysical Research Communications·T F Linsenmayer, M J Hendrix
Apr 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R YangL C Gerstenfeld
Jan 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O Jacenko, R S Tuan
Jan 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O JacenkoR S Tuan
Jan 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B M NeugebauerP V Hauschka
Jan 21, 1995·BMJ : British Medical Journal·J M Bland, D G Altman
Aug 1, 1994·The Journal of Cell Biology·Y IshizakiM C Raff

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Citations

Aug 30, 2008·Cells, Tissues, Organs·Thorsten KirschJeffrey A Winkles
Apr 6, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H D NahT Kirsch
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W J LandisL C Gerstenfeld
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Louis C GerstenfeldThomas A Einhorn
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T KonT A Einhorn
Mar 31, 2005·Current Topics in Developmental Biology·Catherine M CowanBenjamin Wu
May 6, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Thomas AbergJanna Waltimo-Sirén
Nov 21, 1998·Microscopy Research and Technique·N J HickokR S Tuan
Sep 20, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T AizawaL C Gerstenfeld
Sep 13, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L C GerstenfeldT A Einhorn
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M C MeazziniL C Gerstenfeld
Jun 9, 2001·Experimental Biology and Medicine·J J MinguellP Conget
Feb 3, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Karyn A JourdeuilTamara A Franz-Odendaal
Dec 15, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Gabriel L GaleaPhilippa Francis-West
Sep 1, 2000·Osteoarthritis and Cartilage·A M DeLiseR S Tuan

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