Developmental stage-specific expression of the alpha and beta subunits of the HIF-1 protein in the mouse and human fetus

Molecular Genetics and Metabolism
Ashima MadanH J Cohen

Abstract

Erythropoietin (Epo) is a glycoprotein hormone that is the primary regulator of erythropoiesis. Transcription of the Epo gene increases in response to hypoxia or anemia. Epo is synthesized in the liver in fetal life and in the kidney later in gestation. In the mammalian fetus the switch in Epo production from the liver to the kidney occurs in the third trimester. Hypoxia-inducible factor (HIF-1) is a heterodimeric transcription factor consisting of an alpha and beta subunit that binds under hypoxic conditions to an enhancer element in the 3' region of the Epo gene. In order to determine if there is a relationship between expression of HIF-1 alpha and beta subunits with the shift in expression of the Epo gene from the liver to the kidney or with the transitional events occurring at birth we analyzed the expression of these mRNAs in mouse and human fetuses at different stages of gestation. Total RNA was extracted from the brain, heart, kidney, liver, and lungs of mice at P15, P17, and P19 of gestation, from newborn mice at Days 1 and 3, from an adult and an anemic adult mouse as well as from human fetuses at 14-22 weeks of gestation. RNA was analyzed by Northern blot and slot-blot hybridization using appropriate cDNA probes. HIF-...Continue Reading

References

Apr 1, 1992·Physiological Reviews·W Jelkmann
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G L SemenzaS E Antonarakis
Jan 1, 1989·Transfusion·E D Zanjani, J L Ascensao
Jul 1, 1988·The Journal of Clinical Investigation·M J KouryS T Sawyer
Apr 1, 1981·The Journal of Clinical Investigation·E D ZanjaniR C Ash
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Jun 2, 1995·The Journal of Biological Chemistry·A P LevyM A Goldberg
Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G L Wang, G L Semenza
Jun 5, 1996·Biochemical and Biophysical Research Communications·R H WengerM Gassmann
Aug 14, 1996·Biochemical and Biophysical Research Communications·C M WienerG L Semenza

❮ Previous
Next ❯

Citations

Mar 18, 2009·BMC Developmental Biology·Shu-Yuan LiaoEric J Stanbridge
Jan 29, 2005·Acta Physiologica Scandinavica·R I JensenB L Jensen
Jan 21, 2006·Journal of Forensic Sciences·José Blanco PampínRafael Hinojal Fonseca
Feb 3, 2005·International Immunopharmacology·John J Haddad, Hisham L Harb
Mar 6, 2004·The Journal of Thoracic and Cardiovascular Surgery·Igor E KonstantinovGlen S Van Arsdell
Feb 16, 2005·Toxicology and Applied Pharmacology·Tiina M Asikainen, Carl W White
Mar 24, 2007·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Csaba Galambos, Daphne E deMello
Apr 11, 2003·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Monique E De PaepeFrancois I Luks
May 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Conor W SipeMargaret S Saha
Sep 17, 2004·Pediatric Research·Magali ChiralJean Claude David
Sep 21, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Minke van TuylMartin Post
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sun Hee YimFrank J Gonzalez
Jan 13, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yoshiro NaitoTohru Masuyama
Nov 9, 2011·Nutrition Reviews·Fernando E Viteri
Apr 7, 2009·Current Opinion in Hematology·Amélie Montel-HagenNaomi Taylor
May 13, 2009·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Paul A Checchia, Sanjiv K Gandhi
Dec 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Imad ShamsAaron Avivi
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marc HeidbrederPeter Dominiak
Sep 19, 2014·International Journal of Molecular Medicine·Yang-Gyu Park, Sang-Youel Park
Nov 19, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Jessica D de RooijRonald R de Krijger
Dec 24, 2003·The Journal of Experimental Biology·Kendra J Greenlee, Jon F Harrison
May 4, 2005·The Journal of Thoracic and Cardiovascular Surgery·John G ColesGregory Hannigan
Sep 26, 2006·Gene Expression Patterns : GEP·Diego A RojasAriel E Reyes
Aug 10, 2011·Brain & Development·Christina SchneiderRegina Trollmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.