Developmental studies of Anaplasma marginale (Rickettsiales:Anaplasmataceae) in male Dermacentor andersoni (Acari:Ixodidae) infected as adults by using nonradioactive in situ hybridization and microscopy

Journal of Medical Entomology
N GeG L Murphy

Abstract

The development of Anaplasma marginale Theiler was studied in ticks using a nonradioactive in situ hybridization method developed in our laboratory. Male Rocky Mountain wood ticks, Dermacentor andersoni Stiles, were infected intrastadially by allowing them to feed for 7 d on an infected calf (acquisition feeding). The ticks were then removed and held in a humidity chamber for 5 d before being fed on a 2nd susceptible call for 10 d (transmission feeding). Two groups of 10 ticks were collected daily during the 22-d experiment. In one group one-half of each tick was processed and embedded in paraffin and in the other group one-half of each tick was embedded in LR White for in situ hybridization. The companion tick halves from each group were fixed and embedded in Dow Epoxy Resin resin for routine light and electron microscopy. As detected by in situ hybridization on LR White- and paraffin-embedded sections and by microscopy, initial infection of A. marginale in ticks occurred in gut tissues either on the 7th d of acquisition feeding or the 1st d of the held period and infection persisted throughout transmission feeding. The highest number of ticks with gut infection was observed on the 5th d of transmission feeding. Salivary gland...Continue Reading

Citations

Dec 4, 2001·International Journal for Parasitology·J de la FuenteK M Kocan
Oct 23, 2012·Virus Research·Thomas G Burrage
Sep 30, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sergio D RodríguezCarlos A Vega y Murguía
Nov 3, 2010·Transboundary and Emerging Diseases·P Aubry, D W Geale
Mar 18, 2015·PloS One·Geoffrey E LynnUlrike G Munderloh
Apr 1, 2003·Animal Health Research Reviews·Edmour F BlouinKatherine M Kocan
Oct 18, 2000·Infection and Immunity·A F BarbetG H Palmer
Oct 15, 2003·Clinical Microbiology Reviews·Katherine M KocanRoy D Meléndez
Feb 24, 2001·Annals of the New York Academy of Sciences·K M KocanA F Barbet
Oct 9, 2009·Veterinary Parasitology·Katherine M KocanS A Ewing

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