Developmental validation of the ANDE™ rapid DNA system with FlexPlex™ assay for arrestee and reference buccal swab processing and database searching

Forensic Science International. Genetics
Christopher CarneyRichard F Selden

Abstract

A developmental validation was performed to demonstrate reliability, reproducibility and robustness of the ANDE System with the FlexPlex assay, including an integrated Expert System, across a number of laboratories and buccal sample variations. Previously, the related DNAscan™/ANDE 4C Rapid DNA System using the PowerPlex®16 assay and integrated Expert System Software received NDIS approval in March 2016. The enhanced ANDE instrument, referred to as ANDE 6C, and the accompanying 6-dye, 27-locus STR assay, referred to as FlexPlex, have been developed to be compatible with all widely used global loci, including the expanded set of the CODIS core 20 loci. Six forensic and research laboratories participated in the FlexPlex Rapid DNA developmental validation experiments, testing a total of 2045 swabs, including those obtained from 1387 unique individuals. The goal of this extensive and comprehensive validation was to thoroughly evaluate and document the ANDE System and its internal Expert System to reliably genotype reference buccal swab samples in a manner compliant with the FBI's Quality Assurance Standards and the NDIS Operational Procedures. The ANDE System, including automated Expert System analysis, generated reproducible and c...Continue Reading

Citations

Feb 11, 2020·Journal of Forensic Sciences·Rosemary S TuringanRichard F Selden
Jan 28, 2020·Journal of Forensic Sciences·Erica L RomsosPeter M Vallone
Nov 30, 2019·International Journal of Legal Medicine·Rosemary S TuringanRichard F Selden
Jan 2, 2021·Forensic Science International : Synergy·John M Butler, Sheila Willis
Apr 23, 2021·Journal of Forensic Sciences·Nicole HintonKevin P McNamara
May 6, 2021·International Journal of Legal Medicine·Zi-Lin RenJiang-Wei Yan
May 29, 2021·Science·Elizabeth BarnertM Veronica Svetaz
May 16, 2021·Forensic Science International. Genetics·April M ManzellaLilliana I Moreno
Oct 14, 2021·International Journal of Legal Medicine·Jennifer Churchill CihlarBruce Budowle
Jan 4, 2022·Journal of Forensic Sciences·Belinda MartinAdrian Linacre

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