Developmental validation of the PowerPlex(®) Fusion 6C System

Forensic Science International. Genetics
Martin G EnsenbergerDouglas R Storts

Abstract

The PowerPlex(®) Fusion 6C System is a 27-locus, six-dye, multiplex that includes all markers in the expanded CODIS core loci and increases overlap with STR database standards throughout the world. Additionally, it contains two, rapidly mutating, Y-STRs and is capable of both casework and database workflows, including direct amplification. A multi-laboratory developmental validation study was performed on the PowerPlex(®) Fusion 6C System. Here, we report the results of that study which followed SWGDAM guidelines and includes data for: species specificity, sensitivity, stability, precision, reproducibility and repeatability, case-type samples, concordance, stutter, DNA mixtures, and PCR-based procedures. Where appropriate we report data from both extracted DNA samples and direct amplification samples from various substrates and collection devices. Samples from all studies were separated on both Applied Biosystems 3500 series and 6-dye capable 3130 series Genetic Analyzers and data is reported for each. Together, the data validate the design and demonstrate the performance of the PowerPlex(®) Fusion 6C System.

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Citations

Nov 7, 2016·Forensic Science International. Genetics·M van den Berge, T Sijen
Dec 3, 2016·International Journal of Legal Medicine·Weian DuChao Liu
Feb 27, 2017·Journal of Forensic and Legal Medicine·M CaputoD Corach
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Nov 21, 2017·International Journal of Legal Medicine·Micaela PoetschJanine Helmus
Dec 4, 2019·BMC Bioinformatics·Slim KarkarDesmond S Lun
Nov 30, 2018·Forensic Sciences Research·Ana BoavidaFrancisco Corte-Real
Jun 21, 2017·Forensic Science, Medicine, and Pathology·Bruce BudowleFrank R Wendt
Nov 16, 2019·Scientific Reports·Hussain M AlsafiahWilliam Goodwin
Nov 3, 2020·Journal of Forensic Sciences·Jianye Ge, Bruce Budowle
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Jana CmejlovaRadek Cmejla
Apr 10, 2017·Forensic Science International. Genetics·Hiromi BrownSudhir K Sinha
Apr 28, 2021·Scientific Reports·Julianna Kesselring RomeroEdna Sadayo Miazato Iwamura
Apr 4, 2021·Electrophoresis·Dide BoelensBruce R McCord
Jul 16, 2021·Forensic Science International. Genetics·Michael HofreiterDaniel Vanek
Aug 13, 2021·Analytical Chemistry·Cong LiuChuixiu Huang
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan
Sep 23, 2021·International Journal of Legal Medicine·Bowei JiangLagabaiyila Zha

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