Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samples

Forensic Science International. Genetics
Allison HoltRobert L Green

Abstract

The quantification of human genomic DNA is a necessary first step in the DNA casework sample analysis workflow. DNA quantification determines optimal sample input amounts for subsequent STR (short tandem repeat) genotyping procedures, as well as being a useful screening tool to identify samples most likely to provide probative genotypic evidence. To better mesh with the capabilities of newest-generation STR analysis assays, the Quantifiler(®) HP and Quantifiler(®) Trio DNA Quantification Kits were designed for greater detection sensitivity and more robust performance with samples that contain PCR inhibitors or degraded DNA. The new DNA quantification kits use multiplex TaqMan(®) assay-based fluorescent probe technology to simultaneously quantify up to three human genomic targets, allowing samples to be assessed for total human DNA, male contributor (i.e., Y-chromosome) DNA, as well as a determination of DNA degradation state. The Quantifiler HP and Trio Kits use multiple-copy loci to allow for significantly improved sensitivity compared to earlier-generation kits that employ single-copy target loci. The kits' improved performance provides better predictive ability for results with downstream, newest-generation STR assays, and t...Continue Reading

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Citations

Jun 13, 2016·Forensic Science International : Synergy·Elena PilliSimonetta Lambiase
Nov 28, 2017·International Journal of Legal Medicine·Amy S HolmesSheree Hughes-Stamm
Aug 15, 2017·International Journal of Legal Medicine·Masashi KitamuraTatsushi Adachi
Jul 19, 2017·International Journal of Legal Medicine·Ronny HedellPetter Mostad
Oct 11, 2017·International Journal of Legal Medicine·Kelly S GrisedaleSudhir K Sinha
Nov 7, 2019·Journal of Forensic Sciences·Samuel J CornwellMarie S Rye
Sep 18, 2020·Forensic Sciences Research·Matthew C GoldsteinTracey Dawson Cruz
Aug 17, 2019·International Journal of Legal Medicine·Jeongyong KimSi-Keun Lim
Dec 4, 2019·BMC Bioinformatics·Slim KarkarDesmond S Lun
Oct 8, 2020·Journal of Forensic Sciences·Rosane Pérez BaldassoRogério Nogueira de Oliveira
Nov 29, 2020·Legal Medicine·Sarah Aurora HeßRichard Jäger
May 16, 2021·Forensic Science International. Genetics·April M ManzellaLilliana I Moreno
May 18, 2021·Forensic Science International : Synergy·Molina Madhulika EkkaBhargav C Patel
Jun 18, 2021·Forensic Science International. Genetics·Glendon J ParkerZachary C Goecker

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