Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites.

Nucleic Acids Research
Ariane GratiasMireille Bétermier

Abstract

Somatic genome assembly in the ciliate Paramecium involves the precise excision of thousands of short internal eliminated sequences (IESs) that are scattered throughout the germline genome and often interrupt open reading frames. Excision is initiated by double-strand breaks centered on the TA dinucleotides that are conserved at each IES boundary, but the factors that drive cleavage site recognition remain unknown. A degenerate consensus was identified previously at IES ends and genetic analyses confirmed the participation of their nucleotide sequence in efficient excision. Even for wild-type IESs, however, variant excision patterns (excised or nonexcised) may be inherited maternally through sexual events, in a homology-dependent manner. We show here that this maternal epigenetic control interferes with the targeting of DNA breaks at IES ends. Furthermore, we demonstrate that a mutation in the TA at one end of an IES impairs DNA cleavage not only at the mutant end but also at the wild-type end. We conclude that crosstalk between both ends takes place prior to their cleavage and propose that the ability of an IES to adopt an excision-prone conformation depends on the combination of its nucleotide sequence and of additional deter...Continue Reading

References

Jul 27, 2002·Annual Review of Microbiology·Carolyn L Jahn, Lawrence A Klobutcher
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Citations

Dec 12, 2012·Quarterly Reviews of Biophysics·Fred DydaAlison Burgess Hickman
Jun 6, 2013·Genome Biology and Evolution·Francesco CataniaMichael Lynch
Jan 1, 2010·Yi chuan = Hereditas·Zhao-Hui Xie
Aug 14, 2012·International Journal of Evolutionary Biology·Emeline DuboisMireille Bétermier
Aug 2, 2011·Annual Review of Genomics and Human Genetics·Mariusz NowackiLaura F Landweber
Nov 3, 2009·Current Opinion in Microbiology·Mariusz Nowacki, Laura F Landweber
Feb 23, 2012·Biology of the Cell·Robert S CoyneSandra Duharcourt
Aug 26, 2015·Nucleic Acids Research·Diana FerroFrancesco Catania
Mar 12, 2017·Cell·Sarah E AllenMariusz Nowacki
Jul 16, 2015·PLoS Genetics·Kamila Maliszewska-OlejniczakJacek K Nowak
May 29, 2020·Cellular and Molecular Life Sciences : CMLS·Iwona RzeszutekMariusz Nowacki
Jun 25, 2015·Microbiology Spectrum·Mireille Betermier, Sandra Duharcourt

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Methods Mentioned

BETA
PCR

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