PMID: 9178042Apr 1, 1997Paper

Developmentally regulated release of intraretinal neurotrophic factors in vitro

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
R Ary-PiresR Linden

Abstract

The effects of conditioned media either from aggregates or from explants of embryonic chick retinae and of recombinant neurotrophins were tested upon the survival in vitro of ganglion cells in dissociated cell cultures from the retina of newborn rats. Ganglion cells were identified by the detection of retrogradely transported horseradish peroxidase injected bilaterally into the superior colliculus. Conditioned media increased significantly the survival of ganglion cells after 2 days in culture, at a wide range of plating densities, and had no effect upon adhesion of rat retinal cells. Media conditioned by cell ensembles from chick retinae from embryonic day 8 (E8) to E16 had neurotrophic effects. Release of neurotrophic activity peaked at E10 E12, irrespective of the numbers of cells or total concentration of protein in the conditioned media. The active molecules were non-dialyzable and were released either in the presence or in the absence of fetal calf serum. The neurotrophic activity was abolished by trypsinization, and recovered by salting-out with 25 75% ammonium sulfate. NT-4, BDNF and, to a lesser extent, NT-3, increased the survival of ganglion cells in our assay, while NGF had no effect. The data show that chick retina...Continue Reading

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Citations

Dec 14, 2002·Brain Research. Developmental Brain Research·Karen CusatoBenjamin E Reese
Jan 13, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S CubillosL Lima
Aug 5, 2000·Progress in Retinal and Eye Research·R Adler
Feb 5, 1999·Progress in Retinal and Eye Research·R LindenL B Chiarini
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Apr 23, 2005·Progress in Retinal and Eye Research·Rafael LindenMariana S Silveira
Aug 31, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C SternbergR Linden
Dec 19, 2001·Journal of Neurobiology·Ricardo A M ReisRafael Linden
Mar 1, 2019·European Journal of Psychotraumatology·Jurriaan JacobsMichel L A Dückers
Feb 24, 2001·Annals of the New York Academy of Sciences·L B Chiarini, R Linden

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