PMID: 6535647Dec 1, 1984Paper

Developmentally regulated surface structures of teratocarcinoma stem cells studied by mutant cell lines

Cell Differentiation
P Dráber, M Vojtísková

Abstract

Monoclonal antibodies TEC-01, TEC-02, and TEC-03, which define three developmentally regulated antigens TEC-1 (SSEA-1-like), TEC-2, and TEC-3, have been used to isolate and characterize teratocarcinoma stem cell mutants with altered expression of surface glycoconjugates. Mutants lacking TEC-1 antigen have been isolated by exposing mutagenized P19S1801A1 cells to TEC-01 antibody, which was conjugated to the toxin from Ricinus communis. None of the mutants exhibits significant changes in the expression of TEC-3 antigen, but some are defective in the expression of TEC-2 antigen. Analysis of the expression of TEC-1,2,3 antigens in different lectin-resistant F9 and OTF9-63 cell lines has shown that all express TEC-1 antigen, but some lectin-resistant phenotypes exhibit reduction in the expression of TEC-2 and/or TEC-3 antigens. Mutational events in genes regulating the expression of specific glycosyltransferases or glycosidases appear to be the biochemical mechanism regulating the expression of TEC-1 and TEC-2 antigens.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T MuramatsuF Jacob
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Aug 1, 1974·Journal of Cellular Physiology·J M LehmanG B Pierce
Sep 1, 1984·Somatic Cell and Molecular Genetics·P Dráber, P Stanley
Sep 1, 1984·Somatic Cell and Molecular Genetics·P Dráber, P Stanley

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Citations

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P Dráber, P Malý
Jan 1, 1987·Differentiation; Research in Biological Diversity·P KoopmanR G Cotton

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