Developmentally synchronized expression of two Bombyx mori Piwi subfamily genes, SIWI and BmAGO3 in germ-line cells

Biochemical and Biophysical Research Communications
Shinpei KawaokaToru Shimada

Abstract

The Piwi subfamily proteins and their partner, Piwi-interacting RNAs (piRNAs), play an important roles in germ-line development and silencing of selfish DNA elements. To date, however, the developmental expression profiles of the Piwi subfamily genes are poorly known. In this study, we examined the expression profiles of two Bombyx mori Piwi subfamily genes, silkworm Piwi (SIWI) and BmAGO3, which are possible partners of Bombyx piRNA-like small RNAs we recently identified in B. mori germ-line cells. Quantitative reverse transcription-polymerase chain reaction analyses demonstrated that these two genes were abundantly expressed in the larval testis, pupal ovary, and adult eggs, suggesting that they might be involved in spermatogenesis and oogenesis in Bombyx. Notably, developmental expression patterns of SIWI and BmAGO3 were remarkably similar. Collectively, our results suggest that SIWI and BmAGO3 may cooperate in an unknown pathway during the development of B. mori germ-line cells.

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Citations

Dec 22, 2012·Nucleic Acids Research·Shinpei KawaokaSusumu Katsuma
Aug 29, 2013·Biochemistry. Biokhimii︠a︡·I A Olovnikov, A I Kalmykova
Sep 7, 2012·PloS One·Dorota KowalczykiewiczJan Wrzesinski
Aug 21, 2013·Insect Science·Gen-Hong WangQing-You Xia
May 29, 2013·Molecular Reproduction and Development·Sneha Ramesh Mani, Celina E Juliano
Mar 26, 2013·Insect Molecular Biology·L ZhuT Kusakabe
Jun 1, 2010·Archives of Insect Biochemistry and Physiology·Zhen LiaoZhaojun Han
Jan 15, 2015·RNA Biology·Susumu KatsumaTakashi Kiuchi
Sep 20, 2011·Molecular Cell·Shinpei KawaokaYukihide Tomari
Jan 24, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Susumu KatsumaMasashi Iwanaga
Apr 21, 2018·International Journal of Molecular Sciences·Muhammad Salman HameedGuang Yang
May 6, 2020·Insect Science·Min FengJing-Chen Sun
Nov 23, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kazuhiro Sakakibara, Mikiko C Siomi
May 16, 2018·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Susumu KatsumaKen Sahara
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·S J RussellJ LaMarre
Jun 12, 2019·Wiley Interdisciplinary Reviews. RNA·Anna KolliopoulouLuc Swevers
Nov 24, 2020·Archives of Insect Biochemistry and Physiology·Susumu KatsumaMasashi Iwanaga

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