Developments in techniques for the isolation, enrichment, main culture conditions and identification of spermatogonial stem cells

Cytotechnology
Yanan HeDong Wang

Abstract

The in vitro culture system of spermatogonial stem cells (SSCs) provides a basis for studies on spermatogenesis, and also contributes to the development of new methods for the preservation of livestock and animal genetic modification. In vitro culture systems have mainly been established for mouse SSCs, but are lacking for farm animals. We reviewed and analyzed the current progress in SSC techniques such as isolation, purification, cultivation and identification. Based on the published studies, we concluded that two-step enzyme digestion and magnetic-activated cell sorting are fast becoming the main methods for isolation and enrichment of SSCs. With regard to the culture systems, serum and feeders were earlier thought to play an important role in the self-renewal and proliferation of SSCs, but serum- and feeder-free culture systems as a means of overcoming the limitations of SSC differentiation in long-term SSC culture are being explored. However, there is still a need to establish more efficient and ideal culture systems that can also be used for SSC culture in larger mammals. Although the lack of SSC-specific surface markers has seriously affected the efficiency of purification and identification, the transgenic study is help...Continue Reading

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Citations

Jul 13, 2017·In Vitro Cellular & Developmental Biology. Animal·Maryam BaazmAdib Zendedel
Jun 13, 2020·BMC Complementary Medicine and Therapies·Hongjie WangSiyun Niu
Aug 23, 2017·The Journal of Biological Chemistry·Jing LiuRongjia Zhou

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