Deviant Pex3p levels affect normal peroxisome formation in Hansenula polymorpha: high steady-state levels of the protein fully abolish matrix protein import

Yeast
Richard J S BaerendsMarten Veenhuis

Abstract

PEX3 encodes at 52 kDa peroxisomal membrane protein (PMP), essential for peroxisome biogenesis in the yeast Hansenula polymorpha. The relation between Pex3p levels and peroxisome formation was studied in wild type (WT) and delta pex3 strains expressing additional copies of PEX3 under control of a substrate-inducible promoter, namely the strong alcohol oxidase (PAOX) or the weaker amine oxidase (PAMO) promoter. In glucose-grown delta pex3 cells, containing PAOX.PEX3, Pex3p was undetectable and peroxisomes were absent. After induction of these cells on methanol, peroxisomes were rapidly formed. At Pex3p levels up to 7-10 times the values observed in WT controls normal peroxisomes were present. However, at further enhanced Pex3p levels a general matrix protein import defect was observed. This phenomenon was paralleled by aberrant peroxisome assembly and the formation of numerous small vesicles. These vesicles contained Pex3p, together with other H. polymorpha PMPs, but lacked the major matrix proteins which has accumulated in the cytosol. The implications of our results on PEX3 gene regulation and functioning of the peroxisomal matrix protein import machinery in H. polymorpha are discussed.

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Citations

Mar 5, 2014·The Journal of Cell Biology·Kèvin KnoopsIda J van der Klei
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·S T SouthS J Gould
Dec 18, 2009·PloS One·Loknath GidijalaIda J van der Klei
Aug 3, 1999·European Journal of Cell Biology·M SoukupovaG Dodt
Apr 20, 2014·Biochimica Et Biophysica Acta·Chris Williams
Aug 1, 2013·Biochemical and Biophysical Research Communications·Chris Williams, Ida J van der Klei
Dec 2, 2011·FEMS Yeast Research·Ruchi SarayaIda J van der Klei
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P E Purdue, P B Lazarow
Aug 7, 2003·The Journal of Biological Chemistry·Adriana Nívea Leao-HelderMarten Veenhuis
May 16, 2002·The Journal of Biological Chemistry·Gert Jan HaanMarten Veenhuis
Jun 8, 2000·FEMS Microbiology Reviews·R J BaerendsM Veenhuis

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