Device-induced platelet dysfunction in mechanically assisted circulation increases the risks of thrombosis and bleeding

Artificial Organs
Zengsheng ChenZhongjun J Wu

Abstract

Thrombotic and bleeding complications are the major obstacles for expanding mechanical circulatory support (MCS) beyond the current use. While providing the needed hemodynamic support, those devices can induce damage to blood, particularly to platelets. In this study, we investigated device-induced alteration of three major platelet surface receptors, von Willebrand factor (VWF) and associated hemostatic functions relevant to thrombosis and bleeding. Fresh human whole blood was circulated in an extracorporeal circuit with a clinical rotary blood pump (CentriMag, Abbott, Chicago, IL, USA) under the clinically relevant operating condition for 4 hours. Blood samples were examined every hour for glycoprotein (GP) IIb/IIIa activation and receptor loss of GPVI and GPIbα on the platelet surface with flow cytometry. Soluble P-selectin in hourly collected blood samples was measured by enzyme linked immunosorbent assay to characterize platelet activation. Adhesion of device-injured platelets to fibrinogen, collagen, and VWF was quantified with fluorescent microscopy. Device-induced damage to VWF was characterized with western blotting. The CentriMag blood pump induced progressive platelet activation with blood circulating time. Particula...Continue Reading

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Citations

Jan 23, 2020·Artificial Organs·Paul S Malchesky
Aug 24, 2019·Frontiers in Cardiovascular Medicine·Camilla Mains BalleAnne-Mette Hvas
Jun 24, 2020·Bulletin of Mathematical Biology·Liwei WangZhongjun J Wu
Feb 12, 2021·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Shigang WangZhongjun J Wu
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Julien LemétayerLisa Prahl Wittberg
Feb 27, 2021·Journal of Thrombosis and Haemostasis : JTH·Kristin KlaeskeAnna L Meyer

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