Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response

Stem Cells International
Mingyan ZhaoHao Lin

Abstract

The extracellular microvesicles (MVs) are attracting much attention because they are found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator in tailoring the differentiation potential of mesenchymal stem cells (MSCs). However, the effect of DXM on the paracrine signaling of MSCs remains unknown. To this point, we aimed to explore the role of DXM in regulating the paracrine activity of MSCs through evaluating the release and function of MSC-MVs, based on their physicochemical characteristics and support on osteogenic response. Results showed that DXM had no evident impact on the release of MSC-MVs but played a pivotal role in regulating the function of MSC-MVs. MVs obtained from the DXM-stimulated MSCs (DXM-MVs) increased MC3T3 cell proliferation and migration and upregulated Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), and osteopontin (OPN) expression. The repair efficiency of DXM-MVs for femur defects was further investigated in an established rat model. It was found that DXM-MVs accelerated the healing process of bone formation in the defect area. Thus, we conclude that using DXM as stimuli to obtain functional MS...Continue Reading

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Citations

Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Apr 8, 2020·Archives of Disease in Childhood. Fetal and Neonatal Edition·Elizabeth K BakerPeter G Davis
Jun 8, 2021·Bone Reports·Vishnu Priya Murali, Christina A Holmes

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Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
PCR
X-Ray

Software Mentioned

Origin
La Theta
Bruker

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