Dexamethasone and azathioprine promote cytoskeletal changes and affect mesenchymal stem cell migratory behavior

PloS One
Natália SchneiderAna Helena Paz

Abstract

Glucocorticoids and immunosuppressive drugs are commonly used to treat inflammatory disorders, such as inflammatory bowel disease (IBD), and despite a few improvements, the remission of IBD is still difficult to maintain. Due to their immunomodulatory properties, mesenchymal stem cells (MSCs) have emerged as regulators of the immune response, and their viability and activation of their migratory properties are essential for successful cell therapy. However, little is known about the effects of immunosuppressant drugs used in IBD treatment on MSC behavior. The aim of this study was to evaluate MSC viability, nuclear morphometry, cell polarity, F-actin and focal adhesion kinase (FAK) distribution, and cell migratory properties in the presence of the immunosuppressive drugs azathioprine (AZA) and dexamethasone (DEX). After an initial characterization, MSCs were treated with DEX (10 μM) or AZA (1 μM) for 24 hrs or 7 days. Neither drug had an effect on cell viability or nuclear morphometry. However, AZA treatment induced a more elongated cell shape, while DEX was associated with a more rounded cell shape (P < 0.05) with a higher presence of ventral actin stress fibers (P < 0.05) and a decrease in protrusion stability. After 7 days o...Continue Reading

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Citations

Dec 24, 2015·Frontiers in Physiology·Magdalena M SchimkeGünter Lepperdinger
Oct 6, 2015·Gut Pathogens·Maria MaragkoudakiMaria Gazouli
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Methods Mentioned

BETA
GTPases

Software Mentioned

AxioVision
Leica LASAF
PASW Statistics ( SPSS
- GAIT
Image J
PAINT
GraphPad INSTAT
Cellquest

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