Dexamethasone attenuates methacholine-mediated aquaporin 5 downregulation in human nasal epithelial cells via suppression of NF-κB activation

International Forum of Allergy & Rhinology
Yung-Lung ChangShao-Cheng Liu

Abstract

Cholinergic stimulation plays a major role in inflammatory airway diseases. However, its role in airway surface liquid homeostasis and aquaporin 5 (AQP5) regulation remains unclear. In this study we sought to determine the effects of methacholine and dexamethasone on AQP5 expression in human nasal epithelial cells (HNEpC). HNEpC were cultured with methacholine or dexamethasone at 4 concentrations in vitro. The subcellular distribution of AQP5 was explored using immunocytochemistry. The pharmacologic effects of methacholine and dexamethasone on the expression of the phosphorylation of cyclic adenosine monophosphate-responsive element binding protein (p-CREB), AQP5, and nuclear factor-kappaB (NF-κB) were examined using Western blotting. AQP5 was found to be located in cell membrane and cytoplasm and present in every group without a statistically significant difference. Methacholine inhibited expression of AQP5 and p-CREB in HNEpC, whereas dexamethasone increased these protein levels dose-dependently in a statistically significant manner. In turn, HNEpC treated with methacholine and dexamethasone showed the same trends as those intervened separately with these 2 drugs. Moreover, dexamethasone had the ability to reverse the inhibit...Continue Reading

References

Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·J E TowneA G Menon
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·S M KredaS E Gabriel
Apr 2, 2003·Pharmacology & Therapeutics·Fiona R Coulson, Allison D Fryer
Jan 15, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Most Nahid ParvinKazuo Hosoi
Nov 4, 2005·Proceedings of the American Thoracic Society·Kristen E Belmonte
May 11, 2006·Respiratory Research·Reinoud GosensAndrew J Halayko
Nov 29, 2007·Biochemical and Biophysical Research Communications·Janghee WooChulso Moon
Feb 5, 2008·European Journal of Pharmacology·Mirella ProfitaMark Gjomarkaj
Feb 19, 2008·Respiratory Physiology & Neurobiology·Yong BenChunxue Bai
May 17, 2008·American Journal of Respiratory Cell and Molecular Biology·Catherine M McDougallGarry M Walsh
May 27, 2008·British Journal of Pharmacology·I Wessler, C J Kirkpatrick
May 22, 2010·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Weiwei Wang, Ming Zheng
Jun 5, 2010·The American Journal of Pathology·Chenjuan YaoKazuo Hosoi
Sep 30, 2010·Respiratory Research·Tjitske A OenemaReinoud Gosens
Aug 9, 2011·Pulmonary Pharmacology & Therapeutics·Qixin FanZhichao Li
Oct 5, 2013·Biochimica Et Biophysica Acta·Rebecca E DayMatthew T Conner
Mar 18, 2015·American Journal of Rhinology & Allergy·Weiwei WangRuitao Zhang
Jul 26, 2015·Pflügers Archiv : European journal of physiology·A EckhardH Löwenheim
Nov 17, 2015·PloS One·Philip KitchenSusanna Törnroth-Horsefield
Sep 15, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Shao-Cheng LiuHsing-Won Wang

❮ Previous
Next ❯

Citations

Aug 23, 2020·International Journal of Molecular Sciences·Su Young JungSeung Geun Yeo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

American Journal of Rhinology & Allergy
Weiwei WangRuitao Zhang
European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Shao-Cheng LiuHsing-Won Wang
European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Weiwei Wang, Ming Zheng
© 2022 Meta ULC. All rights reserved