PMID: 11312142Apr 20, 2001Paper

Dexamethasone blocks antioestrogen- and oxidant-induced death of pituitary tumour cells

The Journal of Endocrinology
C J NewtonS L Atkin

Abstract

The oestrogen receptor is fundamental to the growth and survival of the rat pituitary tumour cell line, GH(3). Our previous studies have shown that antioestrogens such as RU 58668 and ZM 182780 will reduce the rate of cell division and also induce cell death. Death of these cells in response to antioestrogen treatment appears to be due to a heightened sensitivity to reactive oxygen species (ROS). As part of a study to determine the cross-talk between steroid receptor systems in these cells, we have observed that the glucocorticoid, dexamethasone (Dex), inhibits antioestrogen-induced cell death. Cell death induced by H(2)O(2) is enhanced by ZM 182780 and this effect is also blocked by Dex. As apoptotic cell death in a number of systems involves an early loss of mitochondrial membrane potential (DeltaPsi(m)), we have performed detailed studies on the time-course of DeltaPsi(m) loss in relation to the loss in cell membrane function. These studies have indicated that a loss of DeltaPsi(m) parallels a loss of cell membrane function - this is more characteristic of necrosis than of apoptosis. From microscopic observations of these cells in response to H(2)O(2), it has been noted that early cell membrane blebbing, induced by H(2)O(2),...Continue Reading

Citations

Jan 25, 2003·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·C J NewtonP T McCollum
Feb 15, 2005·Archives of Biochemistry and Biophysics·Seung Hyun JeonSung-Goo Chang
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seonghan KimYoo-Hun Suh

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