Dexamethasone can stimulate G1-S phase transition in human airway fibroblasts in asthma

The European Respiratory Journal
B FoutyMonica Kraft

Abstract

Corticosteroids are the first line of therapy for asthma. Whether they alter the progression of airway remodelling in asthma is, as yet, unknown. To determine whether corticosteroids could alter the fibroblast cell cycle the current authors studied the effect of dexamethasone on cultured airway fibroblasts obtained from nine mild-to-moderate, steroid-naïve asthmatics (forced expiratory volume in one second 78+/-4% predicted), and seven normal controls. Fibroblasts were cultured from endobronchial biopsies obtained via bronchoscopy. Cells were exposed to dexamethasone (10(-9)-10(-7) M) and studied at 72 h to determine differences in progression through the cell cycle. In asthmatic fibroblasts, dexamethasone, at concentrations of 10(-8)M and 10(-7)M, nearly doubled the number of cells in the S phase (17.8+/-3.0% and 18.4+/-3.1%, respectively) compared with untreated fibroblasts (10.3+/-1.4%). There was no significant effect in normal control fibroblasts. Dexamethasone induced hyperphosphorylation of the tumour suppressor, retinoblastoma (RB) in asthmatic fibroblasts; fibroblasts from normal controls had significantly less hyperphosphorylation of RB. No difference in protein expression of the CCAAT/enhancer binding protein alpha b...Continue Reading

Citations

Jul 1, 2014·International Immunopharmacology·Ching-Huei LinDong Chuan Wu
Feb 22, 2011·Pharmacology & Therapeutics·Pierre-Olivier GirodetPatrick Berger
Aug 8, 2007·The Journal of Allergy and Clinical Immunology·Thais MauadPeter J Sterk
May 8, 2007·The Journal of Allergy and Clinical Immunology·Arnaud BourdinPascal Chanez
Dec 19, 2013·Pediatric Pulmonology·Serena PanigadaGiovanni A Rossi
Sep 11, 2010·Respiratory Medicine·Laura PujolsCésar Picado
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Davinder KaurChristopher Brightling
Dec 27, 2012·The World Allergy Organization Journal·Eran PickholtzFrancesca Levi-Schaffer
Jun 1, 2011·The World Allergy Organization Journal·Jia-Wang WangShyam Mohapatra
Apr 6, 2021·Frontiers in Medicine·Byung-Keun KimHeung-Woo Park
Jul 18, 2021·Journal of Neural Transmission·Frank FaltracoJohannes Thome

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

The Journal of Pharmacology and Experimental Therapeutics
Rakesh K KumarPaul S Foster
American Journal of Respiratory and Critical Care Medicine
William R HendersonEmil Y Chi
American Journal of Respiratory and Critical Care Medicine
Reinoud GosensHerman Meurs
© 2021 Meta ULC. All rights reserved