Dexamethasone downregulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification

Circulation Research
John Paul KirtonM Yvonne Alexander

Abstract

Vascular calcification is present in many pathological conditions and is recognized as a strong predictor of future cardiovascular events. Current evidence suggests that it is a regulated process involving inducing and inhibitory molecules. Glucocorticoids have great clinical importance as antiinflammatory drugs and can act as potent inducers of osteogenic differentiation in vitro. The effect of glucocorticoids on vascular cells in vivo remains obscure. Pericytes are pluripotent cells that can differentiate into osteoblasts, and recent evidence suggests that they could participate in vascular calcification. We hypothesized that the synthetic glucocorticoid dexamethasone would enhance the rate of pericyte differentiation and mineralization in vitro with a concomitant suppression of calcification-inhibitory molecules. Three weeks of dexamethasone treatment induced a 2-fold increase in (1) alkaline phosphatase activity, (2) calcium deposition, and (3) the number of nodules formed in vitro; and a reduction in the expression of matrix Gla protein (MGP), osteopontin (OPN), and vascular calcification-associated factor (VCAF) mRNAs. The glucocorticoid receptor antagonist Org 34116 abolished dexamethasone-accelerated pericyte differenti...Continue Reading

References

May 1, 1994·The Journal of Clinical Investigation·K E WatsonL L Demer
Apr 1, 1993·The Journal of Clinical Investigation·K BoströmL L Demer
Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M J DohertyA E Canfield
Dec 31, 1998·Virchows Archiv : an International Journal of Pathology·M JeziorskaD E Wooley
Apr 15, 2000·The Journal of Biological Chemistry·S JonoC M Giachelli
Apr 19, 2000·Zeitschrift für Kardiologie·C M ShanahanP L Weissberg
Mar 6, 2002·The Journal of Biological Chemistry·Elisheva SmithBaruch Frenkel
May 6, 2003·Circulation Research·Georgina CollettAnn E Canfield
May 8, 2003·Journal of Cell Science·Glyn NelsonMichael R H White
Oct 29, 2003·Circulation·Yin TintutLinda L Demer
Oct 31, 2003·Journal of Cellular Biochemistry·Amina F ZebboudjKristina Boström
Dec 23, 2003·Stem Cells·Kelly A PurpuraPeter W Zandstra
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alan GarfinkelLinda L Demer
Jul 10, 2004·Essays in Biochemistry·Udo MoehrenAria Baniahmad
Oct 19, 2004·Biochimica Et Biophysica Acta·Onard J L M SchoneveldWouter H Lamers
May 14, 2005·Circulation Research·G D M Collett, A E Canfield

❮ Previous
Next ❯

Citations

Aug 28, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Takuya UenoToshihide Kumamoto
Jul 17, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Guanghong JiaDevendra K Agrawal
Jun 12, 2009·International Archives of Allergy and Immunology·Masatsugu KurokawaMitsuru Adachi
Nov 21, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Robert Terkeltaub
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Wei XueTeresa Borrás
Mar 4, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Eiji SaitohYoshiyuki Seyama
Nov 7, 2007·European Journal of Endocrinology·Brian R Walker
Jul 11, 2009·The American Journal of Pathology·Susann HelasLorenz C Hofbauer
Jul 23, 2009·Biochemical and Biophysical Research Communications·Xiaohui DuanYongfen Qi
Sep 12, 2013·Stem Cells International·William C W ChenBruno Péault
Aug 8, 2007·Journal of Vascular Surgery·Raul J Guzman
Feb 23, 2010·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·T WillenbergI Baumgartner
Sep 9, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Amanda Fales-WilliamsHeather Flaherty
Oct 6, 2010·Multiple Sclerosis : Clinical and Laboratory Research·Lars BörnsenFinn Sellebjerg
Dec 12, 2017·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Daniel ZicklerRusan Catar
Feb 14, 2018·Journal of the American Heart Association·Isabella AlbaneseAdel Schwertani
Nov 11, 2006·Circulation Research·Rebecca C JohnsonJoseph Loscalzo
May 9, 2009·Circulation Research·M Yvonne Alexander
Sep 13, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael R PreuschFlorian Bea
Jan 20, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Iris Z JaffeMichael E Mendelsohn
Jan 10, 2009·The Journal of Rheumatology·Mark FaheyAnn K Rosenthal
Sep 9, 2017·Future Science OA·Rani da Cunha MorettiSilvio Eduardo Duailibi
Dec 25, 2007·The Journal of Biological Chemistry·Kristen D HadfieldAnn E Canfield
Aug 2, 2019·Current Opinion in Lipidology·Tamer SallamLinda L Demer
Aug 7, 2008·The Journal of Clinical Endocrinology and Metabolism·M J H J DekkerH Tiemeier
Feb 27, 2018·Frontiers in Bioengineering and Biotechnology·Aleksandra Leszczynska, J Mary Murphy
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Emmanuel NwadoziTara L Haas
May 28, 2020·Frontiers in Pharmacology·Yalan LiZhongqun Wang
May 5, 2017·Chinese Medical Journal·Nonanzit Pérez-HernándezJosé Manuel Rodríguez-Pérez
May 4, 2017·Journal of Translational Medicine·Adham Sameer A BardeesiHui Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.