Dexamethasone downregulates the expression of parathyroid hormone-related protein (PTHrP) in mesenchymal stem cells

Steroids
Mikael AhlstromChristel Lamberg-Allardt

Abstract

Parathyroid hormone-related protein (PTHrP) has been shown to have anabolic effects in women with postmenopausal osteoporosis. PTHrP promotes the recruitment of osteogenic cells and prevents apoptotic death of osteoblasts and osteocytes. The receptor responsible for the effects of PTHrP is the common PTH/PTHrP receptor (PTH1R). Glucocorticoids (GC) are commonly used as drugs to treat inflammatory diseases. Long-term GC treatments are often associated with bone loss which can lead to GC-induced osteoporosis. The aim of this work was to study the effects of the glucocorticoid dexamethasone (Dex) on the expression of PTHrP and PTH1R in adult human mesenchymal stem cells, the progenitor cells of osteoblasts. Adult human mesenchymal stem cells (hMSC) were cultured and differentiated by standard methods. The expression of PTHrP and PTH1R mRNA was assayed by real-time qPCR. The PTHrP release into the culture media was measured by an immunoradiometric assay. Treatment with Dex (10 nM) resulted in an 80% drop in the PTHrP release within 6 h. A 24 h Dex treatment also reduced the expression of PTHrP mRNA by up to 90%. The expression of PTH1R receptor mRNA was simultaneously increased up to 20-fold by 10 nM Dex. The effects of Dex on PTHr...Continue Reading

References

Jan 15, 1992·Molecular and Cellular Biochemistry·J MaoM Kalimi
Nov 1, 1990·The Journal of Clinical Endocrinology and Metabolism·S L AsaD J Drucker
Dec 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I YamamotoG V Segre
Jan 1, 1996·Journal of Cellular Physiology·N SudaJ M Moseley
Sep 5, 1996·The New England Journal of Medicine·T J Martin
Oct 23, 1997·European Journal of Endocrinology·I R Reid
Apr 29, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J Yaghoobian, T B Drüeke
Nov 10, 1998·Critical Reviews in Eukaryotic Gene Expression·B Lanske, H M Kronenberg
Dec 30, 1998·Calcified Tissue International·L X BiE Mainous
Aug 17, 1999·The Journal of Clinical Investigation·B LanskeH M Kronenberg
Jul 13, 2002·Annals of the New York Academy of Sciences·Ernesto Canalis, Anne M Delany
Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Mara J HorwitzAndrew F Stewart
Aug 26, 2003·Journal of Cellular Biochemistry·Ryan M PorterAaron S Goldstein
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shilpa ChoudharyCarol C Pilbeam
Sep 3, 2005·The Journal of Clinical Investigation·T John Martin
May 9, 2006·Trends in Endocrinology and Metabolism : TEM·Gherardo MazziottiAndrea Giustina
Nov 16, 2007·The New England Journal of Medicine·Kenneth G SaagRobert Marcus
Feb 6, 2008·Journal of Cellular Physiology·Antonio R G SusperreguiFrancesc Ventura

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Citations

Mar 16, 2011·Biochemical and Biophysical Research Communications·Takashi KajitaniTomoki Okazaki
May 6, 2019·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·H ChandlerB Lanske
Jul 10, 2019·Stem Cells International·Vasilis Paspaliaris, George Kolios

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