Dexamethasone enhances vasopressin-induced aquaporin-2 gene expression in the mpkCCD cells

American Journal of Physiology. Renal Physiology
Kuang-Ting KuoMing-Jiun Yu

Abstract

The mouse cortical collecting duct cell (mpkCCD) has been an instrumental cell model for studying vasopressin-mediated aquaporin-2 regulation. This cell line was first developed by Vandewalle's group from a transgenic mouse carrying the transforming SV40 antigens driven by the pyruvate kinase promoter. To immortalize the cells, four hormone supplements (dexamethasone, epidermal growth factor, insulin, and triiodothyronine) were used to enhance SV40 antigen expression; however, these hormones appear to have various effects on aquaporin-2 gene expression in the cells. Here, we evaluated the effects of each hormone supplement and found that dexamethasone enhanced vasopressin-induced aquaporin-2 gene expression at both mRNA and protein levels in a dose- and time-dependent manner, without affecting mRNA or protein stability. The effects of dexamethasone were attributed largely to enhanced aquaporin-2 mRNA transcription in association with an enhanced aquaporin-2 promoter activity. Dexamethasone did not affect vasopressin-regulated aquaporin-2 phosphorylation and trafficking. In summary, we optimized the conditions to enhance vasopressin-induced endogenous aquaporin-2 gene expression in the mpkCCD cells. By increasing the amount of a...Continue Reading

References

Feb 22, 1978·Pflügers Archiv : European journal of physiology·B M RaysonT Morgan
Jan 1, 1981·Annals of the New York Academy of Sciences·J B WadeS A Lewis
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R DiGiovanniM A Knepper
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·S NielsenM A Knepper
Aug 1, 1996·The American Journal of Physiology·M YasuiA Aperia
Oct 1, 1996·The American Journal of Physiology·M FurunoS Sasaki
Feb 10, 1999·The American Journal of Physiology·G NishimotoA C Nairn
Sep 1, 2000·American Journal of Physiology. Renal Physiology·T SaitoS Sasaki
Dec 14, 2001·Biochemical and Biophysical Research Communications·J K KimR W Schrier
Jan 10, 2002·The Journal of Biological Chemistry·Udo HaslerPierre-Yves Martin
Oct 21, 2004·American Journal of Physiology. Renal Physiology·Mauro BustamanteEric Féraille
Aug 19, 2005·Journal of the American Society of Nephrology : JASN·Yung-Chang ChenRobert W Schrier
Sep 21, 2006·American Journal of Physiology. Renal Physiology·Jason D HoffertMark A Knepper
Nov 4, 2006·Néphrologie & thérapeutique·Daniel Georges Bichet
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert A FentonMark A Knepper
Apr 23, 2008·Pflügers Archiv : European journal of physiology·M Bens, A Vandewalle
Jul 9, 2008·The Journal of Biological Chemistry·Jason D HoffertMark A Knepper
Aug 1, 2008·Journal of the American Society of Nephrology : JASN·Chunling LiRobert W Schrier
Feb 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ming-Jiun YuMark A Knepper
Mar 10, 2012·PloS One·William L RiceHua A Jenny Lu
Oct 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chin-San LooMing-Jiun Yu
Mar 31, 2015·Journal of the American Society of Nephrology : JASN·Jae Wook LeeMark A Knepper
Apr 2, 2015·The New England Journal of Medicine·Mark A KnepperSoren Nielsen
Aug 21, 2015·American Journal of Physiology. Renal Physiology·Julian ArthurHua Jenny Lu
Nov 18, 2015·Journal of the American Society of Nephrology : JASN·Theun de GrootPeter M T Deen
May 10, 2016·Best Practice & Research. Clinical Endocrinology & Metabolism·Daniel G Bichet, Detlef Bockenhauer

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Citations

Aug 18, 2020·American Journal of Physiology. Renal Physiology·Kit Yee WongMing-Jiun Yu

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Methods Mentioned

BETA
transgenic
PCR
transfection

Software Mentioned

Leica LAS - AF

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