Dexamethasone-induced Intra-Uterine Growth Restriction impacts NOSTRIN and its downstream effector genes in the rat mesometrial uterus

Scientific Reports
Shreeta ChakrabortyRupasri Ain

Abstract

Intra-Uterine Growth Restriction (IUGR) is a major cause of fetal and neonatal mortality. Understanding the impact of IUGR on utero-placental gene expression is key to developing effective therapy. In this report we elucidated the impact of IUGR on NOSTRIN and its downstream effector gene expression in the utero-placental compartments. We showed here that induction of IUGR by maternal dexamethasone administration in rats led to up-regulation of NOSTRIN transcript and protein in the mesometrial triangle of the uterus (MG) and not in other utero-placental compartments as compared to control. This was associated with down-regulation of twelve genes and four cytokines that were known to be regulated by NOSTRIN and also required for maintenance of pregnancy. Interestingly, there was remarkable decrease in phosphorylation of RelA transcription factor in the MG during IUGR in line with the fact that the down regulated genes harbour RelA transcription activation domain in their promoters. Furthermore, HIF-1α level was reciprocal to NOSTRIN expression pattern in the mesometrial compartment during IUGR and also in CoCl2 treated endothelial cells. Over-expression of HIF-1α led to a decrease in NOSTRIN levels suggesting inhibition of Nostr...Continue Reading

References

Jul 4, 2001·Nature Reviews. Genetics·J Rossant, J C Cross
Dec 12, 2001·Molecular and Cellular Endocrinology·M L Langdown, M C Sugden
Jan 26, 2002·Pediatric Research·Mirjana Hahn-ZoricLars A Hanson
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kirstin ZimmermannStefanie Oess
Nov 18, 2003·Acta Obstetricia Et Gynecologica Scandinavica·Jose L BarthaRafael Comino-Delgado
Apr 6, 2004·Journal of Reproductive Immunology·Rupasri Ain, Michael J Soares
Nov 4, 2004·Journal of Intensive Care Medicine·Dara Brodsky, Helen Christou
Feb 5, 2005·Seminars in Fetal & Neonatal Medicine·Vandana ChaddhaJohn Kingdom
Apr 26, 2005·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·W XiangR Jiang
Sep 2, 2005·Molecular and Cellular Biology·Lin-Feng ChenWarner C Greene
Oct 8, 2005·Angiogenesis·Berthold Huppertz, Louis L H Peeters
May 23, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xiang LuoWei Wang
Jun 30, 2006·Molecular Biology of the Cell·Kirstin SchillingAnn Icking
Oct 31, 2006·Oncogene·T D Gilmore, M Herscovitch
Nov 21, 2007·Lancet·Gordon C S Smith, Ruth C Fretts
May 30, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Margie ReamDona M Chikaraishi
Nov 26, 2008·Nature Reviews. Cancer·Christian FischerPeter Carmeliet
Dec 18, 2008·Cardiovascular Research·Christian F Rueda-ClausenSandra T Davidge
Apr 29, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wenpei XiangXiaoyan Xu
Apr 15, 2011·Journal of Pregnancy·Mitsuko FuruyaIchiro Aoki
Jul 21, 2011·Nature Immunology·Andrea OeckinghausSankar Ghosh
Nov 24, 2011·Clinical & Developmental Immunology·Raj RaghupathyFawaz Azizieh
Feb 23, 2012·Endocrinology·Kartik ShankarHoracio Gomez-Acevedo
Jul 21, 2012·PloS One·Tai-Ho HungT'sang-T'ang Hsieh
Sep 13, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P M McGowanM J Duffy
Oct 2, 2012·Journal of Obstetrics and Gynaecology of India·Usha Krishna, Sarita Bhalerao
Oct 4, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Stefania LongoM Stronati
Sep 2, 2014·Reproductive Health·Rehana A SalamZulfiqar A Bhutta
Mar 27, 2015·Cell Biology International·Yungyun FangQiang Zhang
Feb 26, 2016·Frontiers in Physiology·Francesca Gaccioli, Susanne Lager
Feb 27, 2017·The Journal of Biological Chemistry·Shreeta Chakraborty, Rupasri Ain

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Citations

Aug 18, 2020·Journal of Developmental Origins of Health and Disease·Kevin LooiKimberley C W Wang
Sep 11, 2020·Life Science Alliance·Sarbani Saha, Rupasri Ain
Jan 11, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keisuke KozaiMichael J Soares

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Methods Mentioned

BETA
PCR
ELISA
immunoprecipitation
transfection
acetylation
Protein Assay

Software Mentioned

NIH ImageJ
ImageJ

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