PMID: 8590451Apr 1, 1995Paper

Dexamethasone induces an increase in intracellular and membrane-associated lipocortin-1 (annexin-1) in rat astrocyte primary cultures

Cellular and Molecular Neurobiology
J D McLeod, C Bolton

Abstract

1. The antiinflammatory actions of glucocorticoid steroids are thought to occur through induction of the protein lipocortin-1 (LC-1; annexin-1). The purpose of the current study was to investigate whether astrocytic LC-1 content was increased in the presence of a synthetic glucocorticoid, dexamethasone. 2. Steroid-induced changes in cellular levels of LC-1 in astrocytes were determined by electrotransfer and immunoblotting techniques. Separate cell fractions were investigated to study the influence of dexamethasone on astroglial LC-1 content. The effect of culture state on LC-1 expression was also examined. 3. Intracellular LC-1 content was found to decrease after initiation of culture, with a substantial rise in both cell proliferation and LC-1 expression occurring after the replenishment of medium containing steroid-free serum. A further increase in intracellular LC-1 occurred upon incubation with dexamethasone. The glucocorticoid-induced change in intracellular LC-1 was a time-dependent event and coincided with an increase in membrane-associated LC-1. 4. The findings in this study indicate that astrocytic LC-1 content is influenced by cell culture conditions and, in the presence of glucocorticoid steroids, the cellular local...Continue Reading

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Sep 5, 1996·Brain Research. Molecular Brain Research·C Suter-Crazzolara, K Unsicker
Feb 24, 1998·Clinical and Experimental Immunology·I HuitingaF J Tilders
Oct 27, 2010·International Immunopharmacology·Lydia SpurrFulvio D'Acquisto
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Aug 28, 2018·AIMS Neuroscience·Pranav Chintamani Joshi, Sugato Benerjee
Sep 11, 2019·Journal of Neuropathology and Experimental Neurology·Masahiro ShijoToru Iwaki

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