PMID: 9178099Jun 15, 1997Paper

Dexamethasone induces rapid actin assembly in human endometrial cells without affecting its synthesis

Journal of Cellular Biochemistry
S B KoukouritakiChristos Stournaras

Abstract

Dexamethasone exerts a stimulatory effect of rapid-onset on the polymerization of actin. This has been documented in human endometrial adenocarcinoma Ishikawa cells, resulting in an acute, dose-dependent decrease in the G/total-actin ratio. In the present study we completely characterized this fast and apparently nongenomic effect of dexamethasone on actin assembly. We followed the morphological alterations of actin cytoskeleton and measured the time-dependent dynamics of actin polymerization both by ruling out any changes of total actin in the cells and by measuring its transcript. Rapid changes in actin polymerization were accurately measured using a highly sensitive and quantitative rhodamine-phalloidin fluorimetric assay. Ishikawa cells, exposed to 0.1 microM dexamethasone for various time periods up to 24 h, showed a highly significant, rapid, and transient increase in the polymerization of actin starting within 15 min of dexamethasone exposure and lasting 2 h. Treated cells showed a significant (1.79-fold) enhancement of the fluorescent signal compared to untreated cells at 15 min. This value decreased continuously in a time-dependent manner, reaching control levels after 120 min and remained so for the next 24 h. Confoca...Continue Reading

References

Nov 1, 1991·The American Journal of Physiology·H F CantielloD A Ausiello
Dec 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·N AntonakisA Gravanis
Jul 1, 1991·The Biochemical Journal·J E Hesketh, I F Pryme
Jan 1, 1991·The American Journal of Physiology·G H DingR M Hays
Dec 1, 1988·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·A SteinerW Vetter
Aug 1, 1986·The Journal of Clinical Endocrinology and Metabolism·A Gravanis, E Gurpide
Sep 15, 1985·The Biochemical Journal·K M RaoM A Virji
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 10, 1984·Biochemistry·H FaulstichC Stournaras
Sep 1, 1994·Current Opinion in Nephrology and Hypertension·J W MillsB A Stanton
Nov 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Mills, L J Mandel
Sep 1, 1993·The American Journal of Physiology·H SimonR M Hays
Jan 1, 1996·Journal of Structural Biology·B SheehanM Yeager
May 1, 1996·Trends in Biochemical Sciences·M Symons
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J BorskiE G Grau
Aug 1, 1996·Trends in Cell Biology·L M Machesky, A Hall

❮ Previous
Next ❯

Citations

Feb 14, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Bernward G Hinkes
Oct 10, 2013·Frontiers in Neuroendocrinology·Chun-Lei JiangJeffrey G Tasker
Nov 25, 2000·Trends in Endocrinology and Metabolism : TEM·R J Borski
Jan 30, 2002·British Journal of Pharmacology·Jamie D CroxtallRod J Flower
May 28, 2002·Journal of Endocrinological Investigation·P J DavisM Wehling
Sep 16, 2005·Endothelium : Journal of Endothelial Cell Research·Dalit RazDavid Elad
Jul 24, 2013·The Journal of Physiology·María Hernández-Alcalá PérezGabriel Mutungi
Jan 22, 2005·Journal of Cellular Biochemistry·J Pablo RodríguezVerónica Cambiazo
Feb 27, 2004·European Journal of Clinical Investigation·H-C TillmannA Schultz
Mar 13, 2014·Cytoskeleton·Christos StournarasFlorian Lang
Jun 12, 2013·Expert Opinion on Therapeutic Targets·Florian LangChristos Stournaras
Jan 28, 2003·American Journal of Obstetrics and Gynecology·Alan H JobeMachiko Ikegami
Jun 19, 1998·Journal of Cellular Biochemistry·E A PapakonstantiC Stournaras
Sep 28, 2012·American Journal of Physiology. Cell Physiology·Evi SchmidEkaterina Shumilina
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maeilena KampaElias Castanas
Apr 30, 2014·Journal of Ophthalmology·Ching-Hsi HsiaoHsin-Yuan Tan
Jun 26, 1998·Journal of Clinical Laser Medicine & Surgery
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R J BorskiW S Tsai
Aug 14, 1999·Progress in Biophysics and Molecular Biology·Y Shimoni
Apr 28, 1999·The Journal of Laboratory and Clinical Medicine·S B Koukouritaki, E A Lianos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.