Dexamethasone inhibits insulin-stimulated recruitment of GLUT4 to the cell surface in rat skeletal muscle

Metabolism: Clinical and Experimental
S P WeinsteinS W Cushman

Abstract

To test the hypothesis that glucocorticoids reduce insulin-stimulated skeletal muscle glucose transport by inhibiting the recruitment of GLUT4 glucose transporters to the cell surface, we determined the effect of glucocorticoid treatment on cell-surface GLUT4 using the impermeant glucose transporter photolabel, 2-N-4-(1-azi-2,2,2-trifluoroethyl)benzoyl-[2-3H]1,3-bis-(D-mann os-4-yloxy)-2-propylamine (ATB-[2-3H]BMPA), and GLUT4 immunoprecipitation. Male Sprague-Dawley rats were treated with dexamethasone ([Dex] 0.9 mg/kg for 2 days) and compared against pair-fed controls. 2-[3H]deoxyglucose (2-[3H]DG) uptake in isolated soleus muscles was measured under conditions in which uptake reflects glucose transport activity. In control muscles, 2-[3H]DG uptake was stimulated eightfold by insulin (20 nmol/L). Dex treatment reduced maximal insulin-stimulated 2-[3H]DG uptake by 48% +/- 4% (mean +/- SEM) and decreased cell-surface (ATB-[2-3H]BMPA-photolabeled) GLUT4 by 48% +/- 3%, despite an increase in total muscle GLUT4 content of 26% +/- 7%. These findings indicate that glucocorticoid-induced inhibition of insulin-stimulated glucose transport in muscle is due to impaired recruitment of GLUT4 to the cell surface.

Citations

Mar 26, 2013·Molecular and Cellular Endocrinology·Taiyi KuoJen-Chywan Wang
Mar 26, 2013·Molecular and Cellular Endocrinology·Adam J Rose, Stephan Herzig
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Taiyi KuoJen-Chywan Wang
Jun 4, 2011·Diabetes, Obesity & Metabolism·E A KauhM Hompesch
Jan 28, 2010·American Journal of Physiology. Endocrinology and Metabolism·Esben S BuhlKitt Falck Petersen
Dec 5, 2009·Journal of Applied Physiology·G Nathan NakkenWilliam W Winder
Dec 3, 2010·The Journal of Clinical Investigation·Rucha PatelCarolyn L Cummins
Jul 28, 2010·Molecular Endocrinology·Terry D HindsEdwin R Sanchez
May 14, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kuan-Hao SuRaymond F Muzic
Mar 28, 2013·The American Journal of the Medical Sciences·Soonho KwonKathie Hermayer
Mar 1, 2012·La Presse médicale·Laura Gagliardi, Claire Le Jeunne
May 14, 2011·Endocrinology and Metabolism Clinics of North America·Erika A Strohmayer, Lawrence R Krakoff
Feb 23, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Adam J RoseStephan Herzig
Mar 20, 2010·Journal of Biomedicine & Biotechnology·Melpomeni PeppaSotirios A Raptis
Jul 13, 2007·Molecular and Cellular Endocrinology·Alexandros Vegiopoulos, Stephan Herzig
Feb 10, 2009·European Journal of Clinical Investigation·D H van RaalteM Diamant
Mar 19, 2014·Frontiers in Neuroendocrinology·Leonidas Panagiotakopoulos, Gretchen N Neigh
Oct 15, 2013·Diabetes/metabolism Research and Reviews·Jessica L Hwang, Roy E Weiss
Oct 10, 2013·Journal of Diabetes·Antonio PerezRicardo Gomez-Huelgas
Apr 7, 2015·The Veterinary Journal·Saskia G A van der DriftRuurd Jorritsma
May 27, 2014·Frontiers in Endocrinology·Jayanthi ManiamMargaret J Morris
Jun 2, 2016·Archives of Biochemistry and Biophysics·Hong GongChun-Lei Jiang
Jul 8, 2000·JPEN. Journal of Parenteral and Enteral Nutrition·M C MooreR E Goldstein
Nov 25, 2017·Nature Communications·Georgia BalsevichMathias V Schmidt
Jun 24, 2004·American Journal of Physiology. Cell Physiology·Michael S RheeAlicia A McDonough
Feb 8, 2018·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Maria J Peláez-JaramilloCarlos O Mendivil
Apr 12, 2007·Neuroendocrinology·Gerardo G PiroliLawrence P Reagan
Dec 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Charlotte CorporeauJacques Delarue
Nov 2, 2006·American Journal of Physiology. Endocrinology and Metabolism·Dake Qi, Brian Rodrigues

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