Abstract
Glucocorticoid hormones (GCs) are able to modulate leukocyte activity. We studied the effect of dexamethasone (DEX) on the expression of CD2, an adhesion molecule involved in T-lymphocyte homing and activation. Results of flow cytometry analysis and immunoprecipitation with anti-CD2 monoclonal antibodies (mAbs) indicated that in vitro treatment with DEX augments CD2 expression in transformed T-cell lines. This effect correlated with a rapid increase in the mRNA and was inhibited by actinomycin-D (AD). The DEX-induced CD2 augmentation was transient, peaked at days 1-2 and returned to the levels of untreated controls at days 3-4. It was a dose-dependent phenomenon, mediated by the GC receptor (GCR), because it was inhibited by the GCR antagonist RU486, and was not induced by other steroids such as testosterone and progesterone. This CD2 modulation could presumably contribute to GC-induced effects on T-cell activity.
References
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B N CronsteinG Weissmann
Oct 1, 1991·Immunology Letters·R E O'HehirJ R Lamb
Jun 3, 1991·Journal of Immunological Methods·I NicolettiC Riccardi
Jun 1, 1991·Current Opinion in Immunology·R M Steinman, J W Young
Aug 2, 1990·Nature·T A Springer
Sep 1, 1988·The Journal of Experimental Medicine·B E BiererS J Burakoff
May 25, 1989·Nature·P MoingeonE L Reinherz
Feb 10, 1989·Cell·M Beato
Jan 1, 1986·Agents and Actions·M Di RosaL Sautebin
Aug 1, 1983·The Journal of Experimental Medicine·R ShimonkevitzH Grey
Jan 1, 1982·Immunological Reviews·T R Cupps, A S Fauci
Dec 1, 1993·Trends in Pharmacological Sciences·P J Barnes, I Adcock
Feb 1, 1994·Immunobiology·G DanneckerD Niethammer
Mar 1, 1993·The Journal of Infectious Diseases·S FurukawaK Yabuta
Jun 1, 1993·Immunology Today·J F Bach
Jan 28, 1994·Cell·A Weiss, D R Littman
Feb 1, 1994·The Journal of Experimental Medicine·F ReF Colotta
Dec 27, 1995·Journal of Immunological Methods·R S DouglasJ S Moore