Dexmedetomidine inhibits LPS-induced inflammatory responses through peroxisome proliferator-activated receptor gamma (PPARγ) activation following binding to α2 adrenoceptors.

European Journal of Pharmacology
Maki FujimotoTakuya Miyawaki

Abstract

Over the past decade, dexmedetomidine (DEX) has been found to possess an anti-inflammatory effect. However, the local anti-inflammatory mechanism of DEX has not been fully clarified. Some intracellular inflammatory pathways lead to negative feedback during the inflammatory process. The cyclooxygenase (COX) cascade synthesizes prostaglandins (PGs) and plays a key role in inflammation, but is known to also have anti-inflammatory properties through an alternative route of a PGD2 metabolite, 15-deoxy-delta-12,14-prostaglandin J2 (15d-PGJ2), and its receptor, peroxisome proliferator-activated receptor gamma (PPARγ). Therefore, we hypothesized that DEX inhibits LPS-induced inflammatory responses through 15d-PGJ2 and/or PPARγ activation, and evaluated the effects of DEX on these responses. The RAW264.7 mouse macrophage-like cells were pre-incubated with DEX, followed by the addition of LPS to induce inflammatory responses. Concentrations of TNFα, IL-6, PGE2, and 15d-PGJ2 in the supernatants of the cells were measured, and gene expressions of PPARγ and COX-2 were evaluated in the cells. Furthermore, we evaluated whether a selective α2 adrenoceptor antagonist, yohimbine or a selective PPARγ antagonist, T0070907, reversed the effects of ...Continue Reading

References

Jan 5, 1996·Biochimica Et Biophysica Acta·H R Herschman
Jun 17, 1999·Nature Medicine·D W GilroyD A Willoughby
Sep 29, 1999·The American Journal of Clinical Nutrition·S D ClarkeX Sha
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R B ClarkS J Padula
May 29, 2000·The Journal of Biological Chemistry·H InoueK Umesono
Oct 25, 2000·Anesthesiology·T Kamibayashi, M Maze
Dec 21, 2000·Trends in Pharmacological Sciences·G J Murphy, J C Holder
Jun 8, 2001·Annual Review of Biochemistry·T M WillsonS A Kliewer
May 4, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Josué PadillaRichard P Phipps
May 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·John S WelchChristopher K Glass
Jan 6, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·G ChinettiB Staels
Dec 22, 2005·Proceedings·R GertlerE N Silvius
Jan 17, 2012·Journal of Advanced Pharmaceutical Technology & Research·Sandeep TyagiSaurabh Sharma
Jan 22, 2014·Anesthesia and Analgesia·Shintaro SukegawaTakuya Miyawaki
Mar 25, 2014·Immunity·Christopher D BuckleyCharles N Serhan
Jul 15, 2015·European Journal of Pharmacology·Yuka HondaTakuya Miyawaki
Aug 29, 2017·Oxidative Medicine and Cellular Longevity·Shiyan PengFeng Xiao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.