Dextran-coated gold nanoparticles for the assessment of antimicrobial susceptibility

Analytical Chemistry
Sudip NathJ Manuel Perez

Abstract

Bacteria rapidly evolve mechanisms to become resistant to antibiotics. Therefore, identifying an effective antibiotic or antibacterial agent and administering it at concentrations that will successfully prevent bacterial growth (antimicrobial susceptibility) is critical for health care decision making and vital for the battle against multi-drug-resistant bacteria. Currently, the determination of antimicrobial susceptibility requires at least 24 h. Herein, we describe a nanoparticle-based antimicrobial susceptibility assay based on the concanavalin A-induced clustering of dextran-coated gold nanoparticles, which sense the presence of available complex carbohydrates in bacterial suspension. Under conditions of bacterial growth inhibition, addition of concanavalin A results in the formation of extensive dextran gold nanoassemblies, which are facilitated by the presence of free carbohydrates in solution and result in large shifts in the surface plasmon band of the nanoparticles. Meanwhile, at conditions of increased bacterial growth, a decrease in the amount of free carbohydrates in solution will occur due to an increased carbohydrate uptake by the proliferating bacteria. This will result in a decrease in the size of the gold nanop...Continue Reading

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Citations

Feb 24, 2011·Journal of the American Chemical Society·Charalambos KaittanisJ Manuel Perez
Dec 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuo Uehara
Apr 8, 2014·Carbohydrate Polymers·Kalipada BankuraDipankar Chattopadhyay
May 31, 2014·Annals of Biomedical Engineering·Ricky Y T ChiuDaniel T Kamei
Dec 26, 2015·Chemical Reviews·Martina DelbiancoPeter H Seeberger
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Aug 30, 2014·Advances in Colloid and Interface Science·Magdalena OćwiejaKatarzyna Kubiak
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Jun 25, 2015·The Journal of Physical Chemistry. B·Arumugam Selva Sharma, Malaichamy Ilanchelian
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Dec 28, 2010·Colloids and Surfaces. B, Biointerfaces·Eleonora MarsichSergio Paoletti
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Jun 14, 2019·Frontiers in Bioengineering and Biotechnology·Ilaria SanzariAlfredo Ambrosone
Feb 14, 2017·Nano Convergence·Julius A Edson, Young Jik Kwon
Nov 26, 2020·Advanced Healthcare Materials·Lana PapafilippouMarilena Hadjidemetriou
Sep 11, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pratigyan DashMamoni Dash
Mar 6, 2010·Chemistry of Materials : a Publication of the American Chemical Society·Sudip NathJ Manuel Perez
Feb 23, 2020·Bioconjugate Chemistry·Kelly ReesW Russ Algar

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