Dextran is resistant to lysosomal digestion in kidney tubules

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
E I Christensen, A B Maunsbach

Abstract

Low molecular weight dextran (Rheomacrodex) was infused into dextran resistant rats in a dose of 5 g/kg body weight. The kidneys were studied by electron microscopy at different time intervals after infusion using a special fixative for the demonstration of dextran. The lysosomes of proximal tubule cells gradually accumulated dextran which remained in small amounts even after 10 days. In separate kidney slice experiments the ability of dextran-loaded proximal tubule lysosomes to digest absorbed proteins was determined using 125I-labelled lysozyme. There were no changes in lysosomal protein digestion. Labelled dextran was resistant to digestion in vitro by homogenates of rat or rabbit kidney cortex or isolated rat lysosomal enzymes. It is concluded that the protein absorption pathway and lysosomal protein catabolism is unchanged after tubular uptake of dextran despite pronounced ultrastructural alterations to the lysosomal system and that dextran is resistant to lysosomal digestion in renal proximal tubules.

References

Sep 1, 1979·Kidney International·E I Christensen, A B Maunsbach
May 1, 1972·The Journal of Cell Biology·N SimionescuG E Palade
Jan 1, 1974·Methods in Enzymology·A B Maunsbach
Dec 1, 1974·Kidney International·E I Christensen, A B Maunsbach
Jul 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·G LITWACK
Apr 1, 1957·Clinica Chimica Acta; International Journal of Clinical Chemistry·E L ROSENFELD, I S LUKOMSKAYA
Jul 5, 1958·Nature·A S McFARLANE
Jun 1, 1963·British Journal of Pharmacology and Chemotherapy·J M HARRIS, G B WEST

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Citations

Jan 1, 1986·The Journal of Membrane Biology·M S Goligorsky, K A Hruska
Feb 1, 1988·Journal of Ultrastructure and Molecular Structure Research·R Bjugn, P R Flood
Feb 26, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Michael DickenmannMichael J Mihatsch
Feb 16, 2005·The Journal of Clinical Investigation·Kiyoshi MoriJonathan Barasch
Dec 16, 2016·BioMed Research International·Norbert Kiss, Péter Hamar
Mar 1, 1983·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·R Hultcrantz

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